ZFIN ID: ZDB-FIG-130719-14 |
Fish: | |
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Observed In: | |
Stage: | Day 6 |
Fig. 3 CagA activates canonical Wnt signaling in the intestinal epithelium. (A–C) Quantitative RT-PCR data showing relative expression levels of the Wnt target genes mycA (A), cyclinD1 (B) and axin2 (C). Expression levels were assayed in dissected adult intestines and normalized to SDHA and β-actin; bars indicate mean ± s.d. of biological triplicates. (D–G) Immunofluorescence micrographs showing proliferating cells (EdU, green, 10-hour label) and cells with nuclear and cytoplasmic accumulation of β-catenin (red staining and white arrowheads) in intestinal cross-sections of wild-type (D), b-cagA (E), axin1tm213 (F) and b-cagA; axin1tm213 (G) animals at 6 dpf. (H) Quantification of proliferating (EdU+) cells. (I) Quantification of cells with nuclear and cytoplasmic accumulation of β-catenin. |
Gene | Antibody | Fish | Conditions | Stage | Anatomy | Assay |
---|---|---|---|---|---|---|
axin2 | WT | standard conditions | Adult | intestine | RTPCR | |
b1231Tg | standard conditions | Adult | intestine | RTPCR | ||
b1232Tg | standard conditions | Adult | intestine | RTPCR | ||
b1233Tg | standard conditions | Adult | intestine | RTPCR | ||
ccnd1 | WT | standard conditions | Adult | intestine | RTPCR | |
b1231Tg | standard conditions | Adult | intestine | RTPCR | ||
b1232Tg | standard conditions | Adult | intestine | RTPCR | ||
b1233Tg | standard conditions | Adult | intestine | RTPCR | ||
myca | WT | standard conditions | Adult | intestine | RTPCR | |
b1231Tg | standard conditions | Adult | intestine | RTPCR | ||
b1232Tg | standard conditions | Adult | intestine | RTPCR | ||
b1233Tg | standard conditions | Adult | intestine | RTPCR |
Antibody | Assay | Fish | Conditions | Stage | Anatomy |
---|---|---|---|---|---|
Ab2-ctnnb1 | IHC | axin1tm213/tm213 | standard conditions | Day 6 | intestinal epithelial cell cytoplasm |
IHC | Day 6 | intestinal epithelial cell nucleus | |||
IHC | axin1tm213/tm213; b1231Tg | standard conditions | Day 6 | intestinal epithelial cell cytoplasm | |
IHC | Day 6 | intestinal epithelial cell nucleus |
Acknowledgments: | ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech. |
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