ZFIN ID: ZDB-FIG-130719-14
Neal et al., 2013 - H. pylori Virulence Factor CagA Increases Intestinal Cell Proliferation by Wnt Pathway Activation in a Transgenic Zebrafish Model. Disease models & mechanisms   6(3):802-10 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes: axin2, ccnd1, myca
Antibody: Ab2-ctnnb1
Fish: WT, axin1tm213/tm213, b1231Tg, b1232Tg, b1233Tg (all 6) expand
Anatomical Terms: intestinal epithelial cell cytoplasm, intestinal epithelial cell nucleus, intestine
Stage Range: Day 6 to Adult
PHENOTYPE:
Fish: axin1tm213/tm213, axin1tm213/tm213; b1231Tg
Observed In: epithelial cell proliferation, intestinal epithelial cell
Stage: Day 6

Fig. 3 CagA activates canonical Wnt signaling in the intestinal epithelium. (A–C) Quantitative RT-PCR data showing relative expression levels of the Wnt target genes mycA (A), cyclinD1 (B) and axin2 (C). Expression levels were assayed in dissected adult intestines and normalized to SDHA and β-actin; bars indicate mean ± s.d. of biological triplicates. (D–G) Immunofluorescence micrographs showing proliferating cells (EdU, green, 10-hour label) and cells with nuclear and cytoplasmic accumulation of β-catenin (red staining and white arrowheads) in intestinal cross-sections of wild-type (D), b-cagA (E), axin1tm213 (F) and b-cagA; axin1tm213 (G) animals at 6 dpf. (H) Quantification of proliferating (EdU+) cells. (I) Quantification of cells with nuclear and cytoplasmic accumulation of β-catenin.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
axin2 WT standard conditions Adult intestine RTPCR
b1231Tg standard conditions Adult intestine RTPCR
b1232Tg standard conditions Adult intestine RTPCR
b1233Tg standard conditions Adult intestine RTPCR
ccnd1 WT standard conditions Adult intestine RTPCR
b1231Tg standard conditions Adult intestine RTPCR
b1232Tg standard conditions Adult intestine RTPCR
b1233Tg standard conditions Adult intestine RTPCR
myca WT standard conditions Adult intestine RTPCR
b1231Tg standard conditions Adult intestine RTPCR
b1232Tg standard conditions Adult intestine RTPCR
b1233Tg standard conditions Adult intestine RTPCR
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-ctnnb1 IHC axin1tm213/tm213 standard conditions Day 6 intestinal epithelial cell cytoplasm
IHC Day 6 intestinal epithelial cell nucleus
IHC axin1tm213/tm213; b1231Tg standard conditions Day 6 intestinal epithelial cell cytoplasm
IHC Day 6 intestinal epithelial cell nucleus
Phenotype Details
Fish Conditions Stage Phenotype
axin1tm213/tm213 standard conditions Day 6 epithelial cell proliferation increased occurrence, abnormal
Day 6 intestinal epithelial cell proliferative, abnormal
axin1tm213/tm213; b1231Tg standard conditions Day 6 epithelial cell proliferation increased occurrence, abnormal
Day 6 intestinal epithelial cell proliferative, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.