ZFIN ID: ZDB-FIG-130719-12
Neal et al., 2013 - H. pylori Virulence Factor CagA Increases Intestinal Cell Proliferation by Wnt Pathway Activation in a Transgenic Zebrafish Model. Disease models & mechanisms   6(3):802-10 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene: EGFP
Fish: b1231Tg, b1232Tg, b1233Tg
Anatomical Terms: intestine, whole organism
Stage: Day 6

Fig. 1 Development of CagA+ transgenic zebrafish. (A) Ubiquitous CagA-EGFP fusion protein expression driven by the b-actin promoter. (B) Ubiquitous CagAEPISA-EGFP fusion protein expression driven by the b-actin promoter. (C) Intestinal CagA-EGFP fusion protein expression driven by the i-fabp promoter. (D) RT-PCR of dissected larval intestine showing expression of cagA and the housekeeping control gene RPL13 at 6 dpf. (E) Quantitative RT-PCR of dissected adult intestines showing relative expression levels of cagA transcript in transgenic lines at 1 year of age. Expression levels were normalized to SDHA and β-actin; bars indicate mean ± s.d. of biological triplicates. Scale bars: 500 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP b1231Tg standard conditions Day 6 whole organism IFL
b1232Tg standard conditions Day 6 intestine IFL
b1233Tg standard conditions Day 6 whole organism IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.