ZFIN ID: ZDB-FIG-130719-13
Neal et al., 2013 - H. pylori Virulence Factor CagA Increases Intestinal Cell Proliferation by Wnt Pathway Activation in a Transgenic Zebrafish Model. Disease models & mechanisms   6(3):802-10 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
PHENOTYPE:
Fish: b1231Tg, b1232Tg, b1233Tg
Observed In: epithelial cell proliferation, intestinal epithelial cell
Stage Range: Day 6 to Days 14-20

Fig. 2 CagA expression causes overproliferation of the intestinal epithelium. (A,B) H&E stained sagittal sections of wild-type (A) and b-cagA transgenic (B) zebrafish intestine at 6 dpf. (C,D) H&E stained sagittal sections of wild-type (C) and b-cagA transgenic (D) zebrafish intestine at 15 dpf. (E,F) Intestinal epithelial cell proliferation at 6 dpf (E) and 15 dpf (F). Bars represent proliferation (mean ± s.e.m.) as a percentage of wild-type; n=10, *P<0.05 using one-way ANOVA with Tukey’s test. (G,H) Total intestinal epithelial cell counts of single H&E stained midline sagittal sections at 6 dpf (G) and 15 dpf (H). (I,J) TUNEL-positive cells in the intestinal epithelium at 6 dpf (I) and 15 dpf (J). Scale bars: 10 μm.

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
b1231Tg standard conditions Day 6 epithelial cell proliferation increased occurrence, abnormal
Day 6 intestinal epithelial cell proliferative, abnormal
Days 14-20 epithelial cell proliferation increased occurrence, abnormal
Days 14-20 intestinal epithelial cell proliferative, abnormal
b1232Tg standard conditions Day 6 epithelial cell proliferation increased occurrence, abnormal
Day 6 intestinal epithelial cell proliferative, abnormal
Days 14-20 epithelial cell proliferation increased occurrence, abnormal
Days 14-20 intestinal epithelial cell proliferative, abnormal
b1233Tg standard conditions Day 6 epithelial cell proliferation increased occurrence, abnormal
Day 6 intestinal epithelial cell proliferative, abnormal
Days 14-20 epithelial cell proliferation increased occurrence, abnormal
Days 14-20 intestinal epithelial cell proliferative, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.