FIGURE

Fig. 5

ID

ZDB-FIG-130220-26

Publication

Henriques et al., 2013 - Telomerase is required for zebrafish lifespan

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Fig. 5

Tissue degeneration is accompanied by p53 induction with a puma acute response and sustained increase of cyclin G1 and cdkn1a expression.

A) Immunoblot analysis of p53 in 10-month old WT and tert^-/- testes and gut lysates. 6 month-old WT zebrafish were injected with the DNA damaging agent doxorubicin to serve as positive control for p53 activation. Asterisk depicts a non-specific cross-reactive band that serves as loading control. RT-qPCR analysis showing expression of B) pro-apoptotic (puma) and cell cycle arrest targets (C) cyclin G1 and D) cdkn1a) in testes, head kidney, gut and muscle of 1, 3, 6 and c.12 months old WT and tert^-/- zebrafish (N = 3 to 8 fish per genotype). Data are represented as mean +/- SEM. Rel. mRNA refers to relative mRNA levels of each gene normalized to beta-actin.

Expression Data

Genes:	bbc3 ccng1 cdkn1a tp53
Antibody:	Ab7-tp53
Fish:	WT tert^{hu3430/hu3430} (AB)
Anatomical Terms:	male organism gut male organism head kidney male organism trunk musculature testis
Stage Range:	Days 30-44 to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tert^{hu3430/hu3430} (AB)
Observed In:	regulation of cell cycle regulation of signal transduction by p53 class mediator
Stage Range:	Days 30-44 to Adult

Phenotype Detail

Acknowledgments

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