ZFIN ID: ZDB-FIG-130220-25
Henriques et al., 2013 - Telomerase is required for zebrafish lifespan. PLoS Genetics   9(1):e1003214 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 4 Proliferative tissue degeneration is accompanied by a sustained decrease in proliferation, acute apoptotic responses, and progressive accumulation of DDR foci.

Representative immunofluorescence images of tissue sections in F1 tert-/- and tert+/+ zebrafish show levels of proliferation (PCNA), apoptosis (TUNEL), DNA damage (53BP1) and senescence-associated β–galactosidase at the ages of 3 to c.12 months. Proliferative tissues such as A) testes, B) head kidney and C) gut sections show sustained significant decrease in proliferation in tert-/- as compared to tert+/+ siblings (panels b, d and e) (p<0.001) and an acute apoptotic response at 3 months of age (p<0.001), which clears by c. 12 months (panels g, i and j). This is accompanied by a progressive increase in 53BP1 foci, reaching maximum significance at c. 12 months (panels l, n and o; p<0.001). This coincides with the presence of senescence-associated β –galactosidase at c.12 months (panels s and t). Note in the testes that most of apoptosis (TUNEL) seems to localize to the spermatogenic zone (Ag, dashed outline) and panel E, where we see an increase in TUNEL-labelled germ cells, labelled with the specific marker PLZF. Most of DNA damage (53BP1) locates to the proliferative zone of maturing spermatocytes (Al, uniform outline). Note in the head kidney B), both the proliferative haematopoietic tissue (Ba–d, arrows) and the non-proliferative mesonephric tubule epithelium (Ba–d, dashed outline) are affected by increased apoptosis (Bg, I, j), DNA damage (Bl, n and o) and senescence (Bs and t) in the tert-/-. D) Muscle, a largely non-proliferative tissue (Da–d) shows significant accumulation of DNA damage foci at in tert-/- by the age of c.12 months (Dn and o; p<0.001), when the muscle fibres are already atrophic (Dn, dashed outline). Quantifications were performed in at least 3 different fields of view of at least 3 different individuals of each genotype at the different time-points indicated in the graphs. Gut IF quantifications were calculated as number of positive cells per “crypt” zone (C) uniform square outline exemplified). Other tissues′ IF was quantified as overall % positive cells. β-galactosidase was quantified as % area stained blue, per field of view. Data are represented as mean +/- SEM. Scale bar = 50 ;mu;m.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-53bp1 IHC WT standard conditions Adult male organism gut
IHC terthu3430/hu3430(AB) standard conditions Adult male organism gut
IHC Adult male organism head kidney
IHC Adult male organism trunk musculature
IHC Adult testis
Ab1-zbtb16 IHC WT standard conditions Adult testis spermatocyte
IHC terthu3430/hu3430(AB) standard conditions Adult testis spermatocyte
Ab2-pcna IHC WT standard conditions Adult testis
IHC terthu3430/hu3430(AB) standard conditions Adult Not Detected testis
Phenotype Details
Fish Conditions Stage Phenotype
terthu3430/hu3430(AB) standard conditions Adult cell division decreased occurrence, abnormal
Adult hematopoietic stem cell homeostasis disrupted, abnormal
Adult intestinal epithelial structure maintenance disrupted, abnormal
Adult male organism has fewer parts of type head kidney hematopoietic multipotent progenitor cell, abnormal
Adult male organism has fewer parts of type head kidney renal tubule, abnormal
Adult male organism gut apoptotic, abnormal
Adult male organism gut senescent, abnormal
Adult male organism head kidney apoptotic, abnormal
Adult male organism head kidney senescent, abnormal
Adult male organism hematopoietic multipotent progenitor cell apoptotic, abnormal
Adult male organism renal tubule apoptotic, abnormal
Adult spermatogonial cell division decreased occurrence, abnormal
Adult testis apoptotic, abnormal
Adult testis senescent, abnormal
Adult testis germ line cell apoptotic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.