header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-130110-28
Dalcq et al., 2012 - RUNX3, EGR1 and SOX9B Form a Regulatory Cascade Required to Modulate BMP-Signaling during Cranial Cartilage Development in Zebrafish. PLoS One   7(11):e50140 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-5

Fig. 8 egr1 and fsta knock-down do not affect ventralisation of cranial neural crest cells.

In situ hybridization was performed at 24 hpf, lateral views, anterior to the left. Scale bars 100 μm. (A,D,G) MOcon 4 ng, (B,E,H) MOegr1 4 ng and (C,F) MOfsta 6 ng. No modification in the expression of markers hand2, edn1 and fsta was observed in MOegr1 4 ng or MOfsta 6 ng injected embryos compared to control.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
edn1 WT control Prim-5 head ventral region ISH
WT + MO1-fsta standard conditions Prim-5 head ventral region ISH
WT + MO3-egr1 standard conditions Prim-5 head ventral region ISH
fsta WT control Prim-5 head ventral region ISH
WT + MO3-egr1 standard conditions Prim-5 head ventral region ISH
hand2 WT control Prim-5 head ventral region ISH
WT + MO1-fsta standard conditions Prim-5 head ventral region ISH
WT + MO3-egr1 standard conditions Prim-5 head ventral region ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One