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ZFIN ID: ZDB-FIG-130110-27
Dalcq et al., 2012 - RUNX3, EGR1 and SOX9B Form a Regulatory Cascade Required to Modulate BMP-Signaling during Cranial Cartilage Development in Zebrafish. PLoS One   7(11):e50140 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Day 4

Fig. 7 Expression of fsta is increased in runx3 and egr1 morphants and sox9b mutants.

Lateral views of in situ hybridizations, anterior to the left. Scale bars 100 μm. (A-F) Compared to controls or wild-type embryos, expression of fsta is up-regulated in egr1 morphants (A,B), runx3 morphants (C,D), and homozygous sox9b mutants (E,F) at 48 hpf. pharyngeal endoderm (pe). (G,H) 4 dpf Alcian Blue stained larvae injected with MOcon 6 ng (K) and MOfsta 6ng (L). Knock-down of fsta causes a hyperplasia of the viscerocranium. Meckel’s cartilage (m), ceratohyal (ch), ceratobranchials 1 to 5 (cb1-5).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
fsta Df(Chr03:sox8b,sox9b)b971/b971 standard conditions Long-pec brain ISH
Long-pec pharyngeal pouch ISH
WT control Long-pec brain ISH
Long-pec pharyngeal pouch ISH
WT + MO1-runx3 standard conditions Long-pec brain ISH
Long-pec pharyngeal pouch ISH
WT + MO3-egr1 standard conditions Long-pec brain ISH
Long-pec pharyngeal pouch ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-fsta standard conditions Day 4 ceratobranchial cartilage hyperplastic, abnormal
Day 4 splanchnocranium hyperplastic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One