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ZFIN ID: ZDB-FIG-121030-25
Hinits et al., 2012 - Zebrafish Mef2ca and Mef2cb are essential for both first and second heart field cardiomyocyte differentiation. Developmental Biology   369(2):199-210 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. S4 Endothelial markers are little affected by Mef2 knockdown.
In situ mRNA hybridisation for mef2cb (A), kdrl (B and C), myl7 (C, red) and cdh5 (E) or immunodetection of MyHC and GFP (D) in wild type or Tg(fli1:GFP) control or injected with mef2d/c MO (except C; mef2cab1086;mef2cbfh288 and sibling embryos). A. Mef2cb mRNA detected in heart (blue arrowhead), telencephalon (white arrowheads), and head vasculature (yellow arrows). B and C. Kdrl (flk-1) expression in mef2d/c morphant and double mef2ca;mef2cb mutant embryos at 21 ss is not changed compare with control and sibling embryos, respectively. D. Expression of endothelial marker fli1:GFP at 24 hpf is similar in control and mef2d/c morphants in the heart and head region (upper panels), but some defects in intersomitic vessels of morphants (lower panels) parallel the somitic muscle MyHC phenotype previously described (Hinits and Hughes, 2007). E. Mild upregulation of cdh5 mRNA in head and heart region (upper panels) and in the trunk region (lower panels) at 24 hpf. lda, lateral dorsal aorta; pmbc, primordial midbrain channel; da, dorsal aorta; pcv, posterior cardinal vein; se, intersegmental vessel. Scale = 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cdh5 WT control Prim-5 head ISH
Prim-5 heart tube ISH
Prim-5 trunk ISH
WT + MO3-mef2d,mef2ca standard conditions Prim-5 head ISH
Prim-5 heart tube ISH
Prim-5 trunk ISH
EGFP twu26Tg standard conditions 20-25 somites heart rudiment IFL
y1Tg control Prim-5 dorsal aorta IFL
Prim-5 intersegmental vessel IFL
Prim-5 lateral dorsal aorta IFL
Prim-5 posterior cardinal vein IFL
Prim-5 primordial midbrain channel IFL
y1Tg + MO3-mef2d,mef2ca standard conditions Prim-5 dorsal aorta IFL
Prim-5 intersegmental vessel IFL
Prim-5 lateral dorsal aorta IFL
Prim-5 posterior cardinal vein IFL
Prim-5 primordial midbrain channel IFL
kdrl WT control 20-25 somites vasculature ISH
WT standard conditions 20-25 somites vasculature ISH
WT + MO3-mef2d,mef2ca standard conditions 20-25 somites vasculature ISH
mef2cab1086/+; mef2cbfh288/+ standard conditions 20-25 somites vasculature ISH
mef2cb twu26Tg standard conditions 20-25 somites head vasculature ISH
20-25 somites heart rudiment ISH
20-25 somites telencephalon ISH
myl7 WT standard conditions 20-25 somites heart rudiment ISH
mef2cab1086/+; mef2cbfh288/+ standard conditions 20-25 somites Not Detected heart rudiment ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-A4.1025 IHC y1Tg control Prim-5 muscle cell
IHC Prim-5 somite
IHC y1Tg + MO3-mef2d,mef2ca standard conditions Prim-5 muscle cell
IHC Prim-5 somite
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg + MO3-mef2d,mef2ca standard conditions Prim-5 intersegmental vessel decreased length, abnormal
Prim-5 intersegmental vessel morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 369(2), Hinits, Y., Pan, L., Walker, C., Dowd, J., Moens, C.B., and Hughes, S.M., Zebrafish Mef2ca and Mef2cb are essential for both first and second heart field cardiomyocyte differentiation, 199-210, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.