ZFIN ID: ZDB-FIG-120907-9
Xing et al., 2012 - Zebrafish foxP2 Zinc Finger Nuclease Mutant Has Normal Axon Pathfinding. PLoS One   7(8):e43968 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage Range: Prim-15 to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage: Protruding-mouth

Fig. 3

foxP2 mutant has normal morphology and CNS patterning.

Whole-mount embryos, brightfield images, scale bar = 50 μm. Lateral views, rostral to the left (A-F); dorsal views, rostral to the top (G, H). (A, B) Gross head morphology is unchanged in mutants at 72hpf. Arrow in (B) shows line used to measure brain size, from the midbrain-hindbrain boundary to the edge of the head by dissecting the midpoint of the lens. (C-D) mRNA expression of foxP2 transcript is unchanged in intensity and pattern in foxP2 mutants. (E-H) in situ expression patterns and intensity of zic2a and dlx2 is unchanged in mutants compared to wild-type embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dlx2a WT standard conditions Prim-15 central nervous system ISH
foxp2zc82/+; foxp2zc83/+ standard conditions Prim-15 central nervous system ISH
foxp2zc82/+; foxp2zc84/+ standard conditions Prim-15 central nervous system ISH
foxp2zc83/+; foxp2zc84/+ standard conditions Prim-15 central nervous system ISH
foxp2 WT standard conditions Long-pec central nervous system ISH
foxp2zc82/+; foxp2zc83/+ standard conditions Long-pec central nervous system ISH
foxp2zc82/+; foxp2zc84/+ standard conditions Long-pec central nervous system ISH
foxp2zc83/+; foxp2zc84/+ standard conditions Long-pec central nervous system ISH
zic2a WT standard conditions Long-pec central nervous system ISH
foxp2zc82/+; foxp2zc83/+ standard conditions Long-pec central nervous system ISH
foxp2zc82/+; foxp2zc84/+ standard conditions Long-pec central nervous system ISH
foxp2zc83/+; foxp2zc84/+ standard conditions Long-pec central nervous system ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
foxp2zc82/+; foxp2zc83/+ standard conditions Protruding-mouth brain size, normal
foxp2zc82/+; foxp2zc84/+ standard conditions Protruding-mouth brain size, normal
foxp2zc83/+; foxp2zc84/+ standard conditions Protruding-mouth brain size, normal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One