ZFIN ID: ZDB-FIG-120907-10
Xing et al., 2012 - Zebrafish foxP2 Zinc Finger Nuclease Mutant Has Normal Axon Pathfinding. PLoS One   7(8):e43968 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Prim-25 to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Fig. 4

foxP2 does not affect neuron specification, apoptosis, or proliferation, but does affect cntnap2 expression levels.

Confocal z-stack images, rostral to the top, scale bars 50 μm. (A, B, E-H, K-L) ventral views. (C, D) dorsal views. (A, B) TH immunohistochemistry at 72hpf shows no difference in WT and mutants in diencephalic dopaminergic neuron pattern or number. (C, D) Calbindin immunohistochemistry in the dorsal hindbrain at 72hpf shows similar patterns and numbers of Purkinje neurons in WT and mutants. (E, F) TUNEL staining for apoptotic cells at 26hpf in the brain shows no difference in pattern or number of cells between WT and mutants. (G, H) Detection of proliferation by H3P staining is similar in WT and mutants at 48hpf. (I, J) Neuropil distribution and intensity visualized with anti-SV2 synaptic vesicle protein antibody at 72hpf is similar in WT and mutants. (K, L) cntnap2 in situ expression pattern at 36hpf shows less expression in mutant embryos, more noticeably in the telencephalon (arrows). (M) quantitative RT-PCR at 72hpf confirms decreased expression of cntnap2 in mutants, which is rescued by injection with full-length foxP2 mRNA (* p<0.05). Y-axis indicates fold-change.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cntnap2b WT standard conditions Prim-25 telencephalon ISH
foxp2zc82/+; foxp2zc84/+ standard conditions Prim-25 telencephalon ISH
th WT standard conditions Protruding-mouth diencephalon dopaminergic neuron IHC
foxp2zc82/+; foxp2zc83/+ standard conditions Protruding-mouth diencephalon dopaminergic neuron IHC
foxp2zc82/+; foxp2zc84/+ standard conditions Protruding-mouth diencephalon dopaminergic neuron IHC
foxp2zc83/+; foxp2zc84/+ standard conditions Protruding-mouth diencephalon dopaminergic neuron IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-SV2 IHC WT standard conditions Protruding-mouth neuron synaptic vesicle
IHC foxp2zc82/+; foxp2zc83/+ standard conditions Protruding-mouth neuron synaptic vesicle
IHC foxp2zc82/+; foxp2zc84/+ standard conditions Protruding-mouth neuron synaptic vesicle
IHC foxp2zc83/+; foxp2zc84/+ standard conditions Protruding-mouth neuron synaptic vesicle
Phenotype Details
Fish Conditions Stage Phenotype
foxp2zc82/+; foxp2zc83/+ standard conditions Prim-5 apoptotic process occurrence, normal
Prim-5 brain structure, normal
Long-pec neural precursor cell proliferation occurrence, normal
Protruding-mouth diencephalon has normal numbers of parts of type dopaminergic neuron, normal
Protruding-mouth diencephalon dopaminergic neuron spatial pattern, normal
Protruding-mouth hindbrain dorsal region has normal numbers of parts of type Purkinje cell, normal
Protruding-mouth hindbrain Purkinje cell spatial pattern, normal
Protruding-mouth synapse assembly process quality, normal
foxp2zc82/+; foxp2zc84/+ standard conditions Prim-5 apoptotic process occurrence, normal
Prim-5 brain structure, normal
foxp2zc82/+; foxp2zc84/+ standard conditions Prim-25 central nervous system development disrupted, abnormal
foxp2zc82/+; foxp2zc84/+ standard conditions Long-pec neural precursor cell proliferation occurrence, normal
Protruding-mouth diencephalon has normal numbers of parts of type dopaminergic neuron, normal
Protruding-mouth diencephalon dopaminergic neuron spatial pattern, normal
Protruding-mouth hindbrain dorsal region has normal numbers of parts of type Purkinje cell, normal
Protruding-mouth hindbrain Purkinje cell spatial pattern, normal
Protruding-mouth synapse assembly process quality, normal
foxp2zc83/+; foxp2zc84/+ standard conditions Prim-5 apoptotic process occurrence, normal
Prim-5 brain structure, normal
Long-pec neural precursor cell proliferation occurrence, normal
Protruding-mouth diencephalon has normal numbers of parts of type dopaminergic neuron, normal
Protruding-mouth diencephalon dopaminergic neuron spatial pattern, normal
Protruding-mouth hindbrain dorsal region has normal numbers of parts of type Purkinje cell, normal
Protruding-mouth hindbrain Purkinje cell spatial pattern, normal
Protruding-mouth synapse assembly process quality, normal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One