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ZFIN ID: ZDB-FIG-120831-13
Eom et al., 2012 - Melanophore Migration and Survival during Zebrafish Adult Pigment Stripe Development Require the Immunoglobulin Superfamily Adhesion Molecule Igsf11. PLoS Genetics   8(8):e1002899 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage: Adult

Fig. 5

igsf11 is expressed by pigment cells and their precursors.

(A,B) In situ hybridization for igsf11 transcript during the larval-to-adult transformation, showing an igfs11-expressing cell near the hypodermis (A, and higher magnification in B). e, epidermis; m, myotome. (C) Similar location to that shown in (B), illustrating a cell within the hypodermis (arrowhead), coexpressing Igsf11 cell (magenta) and mitfa:GFP (green). Nuclei in all immunofluorescence images are counterstained with DAPI (blue). (D,D2) A melanophore isolated in vitro expresses Igsf11 (red). (E). Extra-hypodermal Igsf11+ cells (arrowhead) also coexpressed Igsf11 (magenta) and mitfa:GFP, though some mitfa:GFP+ cells were Igsf11 (lower left of panels). Shown here are cells just ventral to the aorta (a). (F) RT-PCR showed that cell populations isolated by differential centrifugation and highly enriched for melanophores (mel) and xanthophores (xan) express igsf11 transcript. dct, dopachrome tautomerase, expressed by melanophores; aox3, aldehyde oxidase 3, expressed by xanthophores. β-actin, loading control. (G) igsf11 expression was detected in several additional tissue types dissected from adult fish. Scale bars: in (A) 40 μm for (A); in (B) 10 μm for (B); in (C) 10 μm for (C,E); in (D) 20 μm for (D).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
aox5 WT standard conditions Adult xanthophore RTPCR
dct WT standard conditions Adult melanocyte RTPCR
GFP w47Tg standard conditions Adult integument IFL
Adult integument glioblast IFL
igsf11 WT standard conditions Adult brain RTPCR
Adult eye RTPCR
Adult fin RTPCR
Adult heart RTPCR
Adult integument ISH
Adult integument RTPCR
Adult melanocyte ISH
Adult melanocyte RTPCR
Adult ovary RTPCR
Adult testis RTPCR
Adult xanthophore RTPCR
Ab1-igsf11 w47Tg standard conditions Adult integument IHC
Ab1-igsf11 Adult integument glioblast IHC
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.