ZFIN ID: ZDB-FIG-120815-56
Tsai et al., 2012 - The paracrine effect of exogenous growth hormone alleviates dysmorphogenesis caused by tbx5 deficiency in zebrafish (Danio rerio) embryos. Journal of Biomedical Science   19(1):63 Full text @ J. Biomed. Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Term:
Stage: Prim-15
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Prim-15

Fig. 5 Expression of cell cycle-related genes and proteins in tbx5 knockdown and GH-treated zebrafish embryos at 30 h post-fertilization (hpf). Cell cycle-related genes, p27 (A) and cdk2 (B), were significantly induced in MO group embryos throughout the developmental stages but were similar among the WT, WTGH, and MOGH groups. (n = 3, 50 embryos/stage; relative expression = gene expression/β-actin expression). (C-R) Zebrafish embryos was stained with cell cycle-related (Cdk2 and P27) antibodies (red) and counterstained with DAPI (blue) for nuclear observation. In sagittal sections of the heart and transverse sections of the pectoral fins, expression patterns of Cdk2 and P27 were similar in all treatment groups in that they were significantly induced in tbx5-deficienct embryos (E, I, M, Q) and showed insignificant differences among the WT (C, G, K, O), WTGH (D, H, L, P), and MOGH (F, J, N, R) groups. (C-R) The anteriors of the embryos are to the left. WT, wild-type embryos; MO, tbx5 knockdown; MIS, mismatch tbx5-MO-treated embryos; WTGH, WT embryos treated with growth hormone (GH); MOGH, tbx5-MO- and GH-treated embryos. Data are presented as the mean ± S.D. *p < 0.05.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cdk2 WT control Prim-15 whole organism RTPCR
WT + MO1-tbx5a standard conditions Prim-15 whole organism RTPCR
WT + MO1-tbx5a chemical treatment by injection: growth hormone Prim-15 whole organism RTPCR
cdkn1bb WT control Prim-15 whole organism RTPCR
WT + MO1-tbx5a standard conditions Prim-15 whole organism RTPCR
WT + MO1-tbx5a chemical treatment by injection: growth hormone Prim-15 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-tbx5a standard conditions Prim-15 whole organism cdk2 expression increased amount, abnormal
WT + MO1-tbx5a standard conditions Prim-15 whole organism cdkn1bb expression increased amount, abnormal
WT + MO1-tbx5a chemical treatment by injection: growth hormone Prim-15 whole organism cdkn1bb expression amount, ameliorated
Prim-15 whole organism cdk2 expression amount, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Journal of Biomedical Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Biomed. Sci.