ZFIN ID: ZDB-FIG-120810-29
Snyder et al., 2012 - Fbxw7 regulates Notch to control specification of neural precursors for oligodendrocyte fate. Neural Development   7(1):15 Full text @ Neural Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 2

fbxw7 is the gene mutated by the vu56 allele. (A) Schematic representation of genetic mapping and sequencing results. The vu56 mutation mapped to a region of Chromosome 1 containing fbxw7. The vu56 mutation created an amino acid substitution within the second WD repeat of the predicted Fbxw7 protein. (B) Sequence traces from wild-type and vu56 mutant cDNAs. The vu56 mutation changed a G to an A, converting a BamHI restriction site to a HinfI site. (C) RFLP genotyping of homozygous wild type (+/+), heterozygous (+/-) and homozygous mutant (-/-) larvae. U = uncut, B = BamHI digest, H = HinfI digest. (D) RT-PCR analysis of fbxw7 mRNA splicing in control (Crtl) and fbxw7SSMO-injected (MO) 24 hpf embryos and 3 dpf larvae. Upper band in MO lanes (asterisks) indicates splice blocking products. (E) Sequence analysis of RT-PCR products from control embryos, which lack intron 4 sequence (dashed lines) and fbxw7SSMO-injected embryos, which retain intron 4 sequence. Underlined sequence is complementary to the splice-blocking morpholino. (F,G) Lateral images of 3 dpf uninjected control and fbxw7SSMO-injected larvae. Insets show corresponding bright field images. (H-I′) Transverse sections of 3 dpf uninjected control and fbxw7SSMO morpholino injected larvae showing Sox10 expression (red, arrowheads). Panels H′ and I′ show Sox10 labeling merged with olig2:EGFP (green). (J) Quantification of Sox10+ cells per section in control and fbxw7SSMO-injected larvae (n = 8 larvae each genotype; p = 0.0001). Error bars represent SEM.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP vu12Tg standard conditions Protruding-mouth spinal cord glioblast (sensu Vertebrata) IFL
vu12Tg + MO1-fbxw7 standard conditions Protruding-mouth spinal cord glioblast (sensu Vertebrata) IFL
sox10 Ab1-sox10 vu12Tg standard conditions Protruding-mouth spinal cord glioblast (sensu Vertebrata) IHC
Ab1-sox10 Protruding-mouth spinal cord oligodendrocyte IHC
Ab1-sox10 vu12Tg + MO1-fbxw7 standard conditions Protruding-mouth spinal cord glioblast (sensu Vertebrata) IHC
Ab1-sox10 Protruding-mouth spinal cord oligodendrocyte IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
vu12Tg + MO1-fbxw7 standard conditions Protruding-mouth spinal cord dorsal region has extra parts of type glioblast (sensu Vertebrata), abnormal
Acknowledgments:
ZFIN wishes to thank the journal Neural Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neural Dev.