ZFIN ID: ZDB-FIG-120810-31
Snyder et al., 2012 - Fbxw7 regulates Notch to control specification of neural precursors for oligodendrocyte fate. Neural Development   7(1):15 Full text @ Neural Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Protruding-mouth

Fig. 5 Fbxw7 regulates oligodendrocyte number by regulating Notch signaling. (A,B) Transverse sections of 3 dpf wild-type (A) and fbxw7vu56 mutant (B) spinal cords showing Tp1:mCherry and olig2:EGFP expression. Outlined arrowheads mark Tp1:mCherry+, olig2:EGFP+ precursors. Solid arrowheads indicate Tp1:mCherry+, olig2:EGFP+ oligodendrocyte lineage cells. (A′,B′) Tp1:mCherry images alone. (C,D) Confocal z stack projections, from lateral view, of olig2:EGFP and Tp1:mCherry in 6 dpf wild-type sibling and fbxw7vu56 mutant larvae. Arrowheads point to row of olig2:EGFP+ precursors lining the central canal. Outlined arrowheads mark Tp1:mCherry+, olig2:EGFP+ precursors. (E) Relative levels of her4.2 mRNA in 4 dpf wild-type and fbxw7vu56 mutant larvae determined by quantitative PCR. Wild-type level was assigned an arbitrary level of 1.0 (p = 0.0272). (F-N) Lateral images of living 3 dpf larvae treated with DMSO or DAPT plus DMSO from 36–48 hpf. All larvae carried the Tg(olig2:EGFP) reporter to mark OPCs and oligodendrocytes in dorsal spinal cord. DAPT reduced the number of oligodendrocyte lineage cells in larvae of each genotype. Insets show bright field images of larvae. Each larva was genotyped following the experiment.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP fbxw7vu56/+; vu12Tg (AB) control Protruding-mouth glioblast (sensu Vertebrata) IFL
fbxw7vu56/+; vu12Tg (AB) chemical treatment: DAPT Protruding-mouth glioblast (sensu Vertebrata) IFL
fbxw7vu56/vu56; jh11Tg; vu12Tg (AB) standard conditions Protruding-mouth glioblast (sensu Vertebrata) IFL
fbxw7vu56/vu56; vu12Tg (AB) control Protruding-mouth glioblast (sensu Vertebrata) IFL
fbxw7vu56/vu56; vu12Tg (AB) chemical treatment: DAPT Protruding-mouth glioblast (sensu Vertebrata) IFL
jh11Tg; vu12Tg standard conditions Protruding-mouth glioblast (sensu Vertebrata) IFL
vu12Tg control Protruding-mouth glioblast (sensu Vertebrata) IFL
vu12Tg chemical treatment: DAPT Protruding-mouth glioblast (sensu Vertebrata) IFL
mCherry fbxw7vu56/vu56; jh11Tg; vu12Tg (AB) standard conditions Protruding-mouth glioblast (sensu Vertebrata) IFL
Protruding-mouth spinal cord IFL
jh11Tg; vu12Tg standard conditions Protruding-mouth glioblast (sensu Vertebrata) IFL
Protruding-mouth spinal cord IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Neural Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neural Dev.