ZFIN ID: ZDB-FIG-120810-27
Snyder et al., 2012 - Fbxw7 regulates Notch to control specification of neural precursors for oligodendrocyte fate. Neural Development   7(1):15 Full text @ Neural Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 4
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Day 4

Fig. 1 The vu56 mutation causes formation of excess OPCs. (A,B) Lateral images of 3 dpf sibling and vu56 mutants carrying the Tg(olig2:EGFP) reporter marking OPCs in dorsal spinal cord (brackets). Insets show bright field images of living larvae. (C-D′) Transverse sections of sibling and vu56 mutant spinal cords processed for immunohistochemistry to detect Sox10 expression (red, arrowheads). Panels C′ and D′ show Sox10 labeling merged with olig2:EGFP (green). Dashed lines indicate outer edge of spinal cord. (E) Quantification of Sox10+ cells per spinal cord section in wild-type (wt) and vu56 mutant larvae at 3, 4, and 6 dpf (n = 17 wild-type, 19 mutant at 3 dpf (p<0.0001), 7 wild-type and 10 mutant at 4 dpf (p = 0.0002), 5 wild-type and 5 mutant at 6 dpf (p = 0.0357)). (F-I) Transverse sections of 4 dpf wild-type sibling and vu56 mutant larvae, at the level of trunk spinal cord, processed for in situ RNA hybridization to detect plp1a (F,G) and mbp (H, I) expression. Arrow indicates ectopic plp1a expression. (J) Quantification of plp1a+ oligodendrocytes per section in wild-type and vu56 mutant larvae at 4 dpf (n = 4 larvae per genotype; p<0.0001). Error bars represent SEM.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP fbxw7vu56/vu56; vu12Tg(AB) standard conditions Protruding-mouth spinal cord glioblast (sensu Vertebrata) IFL
vu12Tg standard conditions Protruding-mouth spinal cord glioblast (sensu Vertebrata) IFL
mbpa AB standard conditions Day 4 spinal cord proximal surface ISH
fbxw7vu56/vu56(AB) standard conditions Day 4 spinal cord proximal surface ISH
plp1a AB standard conditions Day 4 spinal cord proximal surface ISH
fbxw7vu56/vu56(AB) standard conditions Day 4 spinal cord medial region ISH
Day 4 spinal cord proximal surface ISH
sox10 Ab1-sox10 fbxw7vu56/vu56; vu12Tg(AB) standard conditions Protruding-mouth spinal cord glioblast (sensu Vertebrata) IHC
Ab1-sox10 Protruding-mouth spinal cord oligodendrocyte IHC
Ab1-sox10 vu12Tg standard conditions Protruding-mouth spinal cord glioblast (sensu Vertebrata) IHC
Ab1-sox10 Protruding-mouth spinal cord oligodendrocyte IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
fbxw7vu56/vu56(AB) standard conditions Protruding-mouth swim bladder inflation arrested, abnormal
Protruding-mouth whole organism viability, abnormal
Day 4 oligodendrocyte increased amount, abnormal
Day 4 oligodendrocyte mislocalised medially, abnormal
fbxw7vu56/vu56; vu12Tg(AB) standard conditions Protruding-mouth spinal cord dorsal region has extra parts of type glioblast (sensu Vertebrata), abnormal
Acknowledgments:
ZFIN wishes to thank the journal Neural Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neural Dev.