ZFIN ID: ZDB-FIG-120810-28
Snyder et al., 2012 - Fbxw7 regulates Notch to control specification of neural precursors for oligodendrocyte fate. Neural Development   7(1):15 Full text @ Neural Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 4 Excess OPCs arise from ventral spinal cord precursors in fbxw7vu56 mutant larvae. (A-C′′) Images of spinal cords, from lateral orientation, captured from time-lapse movies of fbxw7+/+ (A-A′′), fbxw7+/- (B-B′′) and fbxw7-/- (C-C′′) larvae carrying the Tg(olig2:EGFP) transgene to mark migrating OPCs. Dorsal is up in all images. Numbers in each panel indicate time (hours, minutes) post fertilization. Red circles mark newly formed OPCs initiating migration from ventral spinal cord. (D) Quantification of OPCs that migrated dorsally in fbxw7+/+ (n = 6), fbxw7+/- (n = 8) and fbxw7-/- (n = 3) larvae from 48 to 63 hpf. (E) Average number of OPC divisions in each genotype. (F,G) Transverse sections of 3 dpf spinal cords labeled with anti-Isl antibody to mark motor neurons (red) in combination with olig2:EGFP expression (green). (H) Quantification of Isl+ cells per section in wild-type sibling and fbxw7-/- larvae (n = 19 wild-type and 17 mutant larvae; p = 0.7658). (I,J) Transverse sections of 3 dpf spinal cords labeled with anti-Sox2 antibody to mark neural precursors (red) in combination with olig2:EGFP expression (green). Oligodendrocyte lineage cells also expressed Sox2 (arrows). Quantification of total spinal cord Sox2+ (K) and Sox2+olig2:EGFP+ (L) cells in wild-type and mutant larvae (n = 17 wild-type and 16 mutant larvae; p<0.0001). (M,N) Transverse sections of 3 dpf spinal cords labeled to detect BrdU incorporation (red) in combination with olig2:EGFP expression (green). Quantification of total spinal cord BrdU+ (O) and BrdU+olig2:EGFP+ (P) cells per section in wild-type sibling and fbxw7-/- larvae (n = 18 wild-type and 15 mutant larvae; p<0.0001). Error bars represent SEM.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP fbxw7vu56/+; vu12Tg(AB) standard conditions Long-pec spinal cord glioblast (sensu Vertebrata) IFL
fbxw7vu56/vu56; vu12Tg(AB) standard conditions Long-pec spinal cord glioblast (sensu Vertebrata) IFL
Protruding-mouth glioblast (sensu Vertebrata) IFL
vu12Tg standard conditions Long-pec spinal cord glioblast (sensu Vertebrata) IFL
Protruding-mouth glioblast (sensu Vertebrata) IFL
sox2 Ab3-sox2 fbxw7vu56/vu56; vu12Tg(AB) standard conditions Protruding-mouth neuroblast (sensu Vertebrata) IHC
Ab3-sox2 vu12Tg standard conditions Protruding-mouth neuroblast (sensu Vertebrata) IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-isl IHC fbxw7vu56/vu56; vu12Tg(AB) standard conditions Protruding-mouth motor neuron
IHC vu12Tg standard conditions Protruding-mouth motor neuron
Phenotype Details
Fish Conditions Stage Phenotype
fbxw7vu56/+; vu12Tg(AB) standard conditions Long-pec spinal cord dorsal region has extra parts of type glioblast (sensu Vertebrata), abnormal
fbxw7vu56/vu56; vu12Tg(AB) standard conditions Long-pec spinal cord dorsal region has extra parts of type glioblast (sensu Vertebrata), abnormal
Protruding-mouth glioblast (sensu Vertebrata) increased amount, abnormal
Protruding-mouth motor neuron normal amount, normal
Protruding-mouth neuroblast (sensu Vertebrata) increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Neural Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neural Dev.