header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-120525-32
Jia et al., 2012 - Protein Phosphatase 4 Cooperates with Smads to Promote BMP Signaling in Dorsoventral Patterning of Zebrafish Embryos. Developmental Cell   22(5):1065-1078 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Shield
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Shield

Fig. 3

Genetic Interactions between Ppp4c and Components of the BMP Signaling Pathway (A and B) Marker alterations at the shield stage caused by 10 pg bmp2b mRNA or 1 ng ppp4cb-MO1 (bMO1) were mutually inhibited by their coinjection. gsc, dorsal views; gata2 and eve1, animal-pole views with dorsal to the right; foxi1, lateral views with dorsal to the right. Statistical data for marker expression are shown in (B). Blue, red, and yellow indicated normal, increased, and decreased expression levels, respectively. The number of observed embryos was indicated. (C–H) ppp4cb-MO1 (bMO1, 1 ng)-induced elongated shape at the bud stage was rescued by coinjection of bmp2b (10 pg) or casmad5 mRNA (100 pg) encoding constitutively active Smad5. See also Figure S3.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
eve1 WT standard conditions Shield whole organism ventral region ISH
WT + MO1-ppp4cb standard conditions Shield whole organism ventral region ISH
foxi1 WT standard conditions Shield whole organism ventral region ISH
WT + MO1-ppp4cb standard conditions Shield whole organism ventral region ISH
gata2a WT standard conditions Shield whole organism ventral region ISH
WT + MO1-ppp4cb standard conditions Shield whole organism ventral region ISH
gsc WT standard conditions Shield whole organism dorsal region ISH
WT + MO1-ppp4cb standard conditions Shield whole organism dorsal region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-ppp4cb standard conditions Shield whole organism elongated, abnormal
Shield whole organism wholly dorsalized, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 22(5), Jia, S., Dai, F., Wu, D., Lin, X., Xing, C., Xue, Y., Wang, Y., Xiao, M., Wu, W., Feng, X.H., and Meng, A., Protein Phosphatase 4 Cooperates with Smads to Promote BMP Signaling in Dorsoventral Patterning of Zebrafish Embryos, 1065-1078, Copyright (2012) with permission from Elsevier. Full text @ Dev. Cell