Fig. 3

Convergent extension and axial defects in Zic3 morphant zebrafish. (A–D) WISH of no tail expression in the notochord at 90% epiboly (A, B) and tailbud (C, D) stages. Control (CTRL) embyos (A, C) showed normal notochord development, whereas Zic3 morphants (B, D) exhibited a broader notochord. (E, F) WISH of myod and dlx3b at the 4-somite stage to label somites and the neural plate border, respectively. The red lines mark the width of the neural plate and the white lines indicate somite width. (G) Average width of the neural plate (measured at the red line) in control and Zic3 morphant embryos. (H,I) Live control (H) and Zic3 morphant (I) embryos at the 12-somite stage. The red lines indicate head-to-tail distance. (J) Average head-to-tail distances in control and Zic3 morphants. Live control (K) and Zic3 morphant (L) embryos at 2 days post-fertilization. Error bars represent SEM. **p < 0.01 by t-test.