ZFIN ID: ZDB-FIG-111101-33
Swartz et al., 2011 - Examination of a palatogenic gene program in zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists   240(9):2204-2220 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 5

Fig. 8

Loss of signaling molecules and transcription factors important in amniote palatogenesis causes craniofacial defects in zebrafish. A–L: Alcian blue and Alizarin red stained the cartilages and bones, respectively, of 5 days postfertilization (dpf) zebrafish. (A: Whole-mount lateral and (B) ventral views of an uninjected control craniofacial skeleton (arrowhead, palate; black arrow, Meckel′s cartilage). C: Flat mounted neurocranium. The anterior neurocranium or zebrafish palate is anterior to the dashed lines. A: Inset is a magnified view showing the three pharyngeal teeth present at this stage. D–F: fgf10a morpholino-injected fish have severely misshaped jaws and palate. D,E: Lateral and ventral views highlight the shortened ethmoid plate and jaw. F: Flatmount view of the neurocranium reveals the shortened ethmoid plate and misshaped trabeculae (arrowhead) and parasphenoid bone (asterisk). G–I: tgfb2 knockdown also causes shortening and misshaping of the jaw, ethmoid plate, trabeculae and parasphenoid. I: Misshaped shortened palate marked by arrowhead, misshaped parsasphenoid marked by asterisk. J–L: pax9a morpholino injection causes defects of the jaw, hyomandibular cartilage, palatal skeleton and teeth. J: Inset, shows the reduction in the number of teeth. K: Lateral view of flat mounted first and second arch-derived pharyngeal skeletal elements, control, (top half of panel) and pax9a morpholino-injected embryos (bottom half of panel). The second arch hyomandibular cartilage is reduced to a rod-like structure in this example (line). L: The palatal skeleton fails to fuse to the posterior neurocranium, arrowhead. Anterior is to the left in all panels. e, eye; ep, ethmoid plate; t, trabeculae.

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-pax9 standard conditions Day 5 ceratobranchial 5 tooth decreased amount, abnormal
Day 5 ethmoid cartilage chondrocyte disorganized, abnormal
Day 5 hyomandibula bifurcated, abnormal
Day 5 hyomandibula cylindrical, abnormal
Day 5 hyomandibula decreased size, abnormal
Day 5 neurocranium decreased length, abnormal
Day 5 parasphenoid shape, abnormal
Day 5 trabecula cranii unfused from neurocranium posterior region, abnormal
Day 5 trabecula cranii chondrocyte disorganized, abnormal
AB + MO1-tgfb2 standard conditions Day 5 ethmoid cartilage decreased length, abnormal
Day 5 ethmoid cartilage shape, abnormal
Day 5 mandibular arch skeleton decreased length, abnormal
Day 5 mandibular arch skeleton shape, abnormal
Day 5 Meckel's cartilage decreased size, abnormal
Day 5 palate decreased size, abnormal
Day 5 palate shape, abnormal
Day 5 parasphenoid decreased length, abnormal
Day 5 parasphenoid shape, abnormal
Day 5 trabecula cranii decreased length, abnormal
Day 5 trabecula cranii shape, abnormal
AB + MO3-fgf10a standard conditions Day 5 ethmoid cartilage decreased length, abnormal
Day 5 mandibular arch skeleton decreased length, abnormal
Day 5 mandibular arch skeleton shape, abnormal
Day 5 Meckel's cartilage shape, abnormal
Day 5 palate shape, abnormal
Day 5 palate square, abnormal
Day 5 palatoquadrate cartilage shape, abnormal
Day 5 parasphenoid decreased length, abnormal
Day 5 parasphenoid shape, abnormal
Day 5 trabecula cranii decreased length, abnormal
Day 5 trabecula cranii shape, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.