ZFIN ID: ZDB-FIG-110909-13
Poulain et al., 2011 - Interplay between Wnt2 and Wnt2bb controls multiple steps of early foregut-derived organ development. Development (Cambridge, England)   138(16):3557-68 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Fig. 2

Wnt2 compensates for loss of Wnt2bb function in prt/wnt2bb mutants. (A-H) Expression of hhex at 28 hpf (A-D) and ceruloplasmin at 48 hpf (E-H) shows a reduction of liver progenitors (bracket) in embryos lacking either wnt2 (C,G) or wnt2bb (B,F) and an absence in MO-wnt2;prt/wnt2bb embryos (D,H), whereas the dorsal pancreas is unaffected (asterisk). (I-N2) Prox1 expression in Tg(XlEef1a1:GFP)s854 embryos at 52 hpf shows smaller livers in MO-wnt2 (J,J2) and prt/wnt2bb embryos (K,K2), and an increasing reduction of the liver in MO-wnt2;prt/wnt2bb embryos (L-N2), which in more severe cases includes a reduction of 2F11-positive EHPD (d:M-N2); distribution of phenotypes indicated as percentages (n=65). (O-R2) Tg(fabp10:dsRed)gz4 expression confirms the reduction of differentiating liver tissue in prt/wnt2bb embryos (P,P2) and MO-wnt2 (Q,Q2) and absence in MO-wnt2;prt/wnt2bb embryos (R,R2) at 74 hpf, indicating that the liver defect fails to recover; Tg(XlEef1a1:GFP)s854 and Tg(ela3l:EGFP)gz2) expression indicates the digestive tract and exocrine pancreas remain unaffected (O-R2). (A-H) Dorsal views and (I-R2) ventral views of projections of confocal stacks; all anterior towards the top. gb, gall bladder; L, liver; p, pancreas.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cp WT control Long-pec liver ISH
WT + MO3-wnt2 standard conditions Long-pec liver ISH
wnt2bbs403/s403 control Long-pec liver ISH
wnt2bbs403/s403 + MO3-wnt2 standard conditions Long-pec Not Detected liver ISH
wnt2bbs404/s404 control Long-pec liver ISH
wnt2bbs404/s404 + MO3-wnt2 standard conditions Long-pec Not Detected liver ISH
DsRed gz2Tg; gz4Tg; s854Tg control Protruding-mouth liver IHC
gz2Tg; gz4Tg; s854Tg + MO3-wnt2 standard conditions Protruding-mouth liver IHC
wnt2bbs403/s403; gz2Tg; gz4Tg; s854Tg control Protruding-mouth liver IHC
wnt2bbs403/s403; gz2Tg; gz4Tg; s854Tg + MO3-wnt2 standard conditions Protruding-mouth Not Detected liver IHC
wnt2bbs404/s404; gz2Tg; gz4Tg; s854Tg control Protruding-mouth liver IHC
wnt2bbs404/s404; gz2Tg; gz4Tg; s854Tg + MO3-wnt2 standard conditions Protruding-mouth Not Detected liver IHC
EGFP gz2Tg; gz4Tg; s854Tg control Protruding-mouth exocrine pancreas IHC
gz2Tg; gz4Tg; s854Tg + MO3-wnt2 standard conditions Protruding-mouth exocrine pancreas IHC
wnt2bbs403/s403; gz2Tg; gz4Tg; s854Tg control Protruding-mouth exocrine pancreas IHC
wnt2bbs403/s403; gz2Tg; gz4Tg; s854Tg + MO3-wnt2 standard conditions Protruding-mouth exocrine pancreas IHC
wnt2bbs404/s404; gz2Tg; gz4Tg; s854Tg control Protruding-mouth exocrine pancreas IHC
wnt2bbs404/s404; gz2Tg; gz4Tg; s854Tg + MO3-wnt2 standard conditions Protruding-mouth exocrine pancreas IHC
GFP gz2Tg; gz4Tg; s854Tg control Protruding-mouth digestive system endoderm IHC
gz2Tg; gz4Tg; s854Tg + MO3-wnt2 standard conditions Protruding-mouth digestive system endoderm IHC
s854Tg control Long-pec liver IHC
Long-pec pancreas IHC
s854Tg + MO3-wnt2 standard conditions Long-pec liver IHC
Long-pec pancreas IHC
wnt2bbs403/s403; gz2Tg; gz4Tg; s854Tg control Protruding-mouth digestive system endoderm IHC
wnt2bbs403/s403; gz2Tg; gz4Tg; s854Tg + MO3-wnt2 standard conditions Protruding-mouth digestive system endoderm IHC
wnt2bbs403/s403; s854Tg control Long-pec liver IHC
Long-pec pancreas IHC
wnt2bbs403/s403; s854Tg + MO3-wnt2 standard conditions Long-pec Not Detected liver IHC
Long-pec pancreas IHC
wnt2bbs404/s404; gz2Tg; gz4Tg; s854Tg control Protruding-mouth digestive system endoderm IHC
wnt2bbs404/s404; gz2Tg; gz4Tg; s854Tg + MO3-wnt2 standard conditions Protruding-mouth digestive system endoderm IHC
wnt2bbs404/s404; s854Tg control Long-pec liver IHC
Long-pec pancreas IHC
wnt2bbs404/s404; s854Tg + MO3-wnt2 standard conditions Long-pec Not Detected liver IHC
Long-pec pancreas IHC
hhex WT control Prim-5 liver primordium ISH
Prim-5 posterior pancreatic bud ISH
WT + MO3-wnt2 standard conditions Prim-5 liver primordium ISH
Prim-5 posterior pancreatic bud ISH
wnt2bbs403/s403 control Prim-5 liver primordium ISH
Prim-5 posterior pancreatic bud ISH
wnt2bbs403/s403 + MO3-wnt2 standard conditions Prim-5 Not Detected liver primordium ISH
Prim-5 posterior pancreatic bud ISH
wnt2bbs404/s404 control Prim-5 liver primordium ISH
Prim-5 posterior pancreatic bud ISH
wnt2bbs404/s404 + MO3-wnt2 standard conditions Prim-5 Not Detected liver primordium ISH
Prim-5 posterior pancreatic bud ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab-2F11 IHC gz2Tg; gz4Tg; s854Tg control Protruding-mouth extrahepatic duct
IHC Protruding-mouth gall bladder
IHC gz2Tg; gz4Tg; s854Tg + MO3-wnt2 standard conditions Protruding-mouth extrahepatic duct
IHC Protruding-mouth gall bladder
IHC s854Tg control Long-pec hepatic duct
IHC s854Tg + MO3-wnt2 standard conditions Long-pec hepatic duct
IHC wnt2bbs403/s403; gz2Tg; gz4Tg; s854Tg control Protruding-mouth extrahepatic duct
IHC Protruding-mouth gall bladder
IHC wnt2bbs403/s403; gz2Tg; gz4Tg; s854Tg + MO3-wnt2 standard conditions Protruding-mouth Not Detected extrahepatic duct
IHC Protruding-mouth Not Detected gall bladder
IHC wnt2bbs403/s403; s854Tg control Long-pec hepatic duct
IHC wnt2bbs403/s403; s854Tg + MO3-wnt2 standard conditions Long-pec hepatic duct
IHC wnt2bbs404/s404; gz2Tg; gz4Tg; s854Tg control Protruding-mouth extrahepatic duct
IHC Protruding-mouth gall bladder
IHC wnt2bbs404/s404; gz2Tg; gz4Tg; s854Tg + MO3-wnt2 standard conditions Protruding-mouth Not Detected extrahepatic duct
IHC Protruding-mouth Not Detected gall bladder
IHC wnt2bbs404/s404; s854Tg control Long-pec hepatic duct
IHC wnt2bbs404/s404; s854Tg + MO3-wnt2 standard conditions Long-pec hepatic duct
Ab3-prox1 IHC s854Tg control Long-pec exocrine pancreas
IHC Long-pec liver
IHC s854Tg + MO3-wnt2 standard conditions Long-pec exocrine pancreas
IHC Long-pec liver
IHC wnt2bbs403/s403; s854Tg control Long-pec exocrine pancreas
IHC Long-pec liver
IHC wnt2bbs403/s403; s854Tg + MO3-wnt2 standard conditions Long-pec exocrine pancreas
IHC Long-pec Not Detected liver
IHC wnt2bbs404/s404; s854Tg control Long-pec exocrine pancreas
IHC Long-pec liver
IHC wnt2bbs404/s404; s854Tg + MO3-wnt2 standard conditions Long-pec exocrine pancreas
IHC Long-pec Not Detected liver
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO3-wnt2 standard conditions Prim-5 liver decreased size, abnormal
Long-pec liver decreased size, abnormal
gz2Tg; gz4Tg; s854Tg + MO3-wnt2 standard conditions Protruding-mouth liver decreased size, abnormal
s854Tg + MO3-wnt2 standard conditions Long-pec liver decreased size, abnormal
wnt2bbs403/s403 control Prim-5 liver decreased size, abnormal
Long-pec liver decreased size, abnormal
wnt2bbs403/s403 + MO3-wnt2 standard conditions Prim-5 liver absent, abnormal
Prim-5 liver decreased size, abnormal
Long-pec liver absent, abnormal
Long-pec liver decreased size, abnormal
wnt2bbs403/s403; gz2Tg; gz4Tg; s854Tg control Protruding-mouth extrahepatic duct decreased size, abnormal
Protruding-mouth gall bladder absent, abnormal
Protruding-mouth gall bladder decreased size, abnormal
Protruding-mouth liver decreased size, abnormal
Protruding-mouth liver development having decreased processual parts cell population proliferation, abnormal
Protruding-mouth liver hypoplastic, abnormal
wnt2bbs403/s403; gz2Tg; gz4Tg; s854Tg + MO3-wnt2 standard conditions Protruding-mouth extrahepatic duct absent, abnormal
Protruding-mouth extrahepatic duct decreased size, abnormal
Protruding-mouth gall bladder absent, abnormal
Protruding-mouth gall bladder decreased size, abnormal
Protruding-mouth liver absent, abnormal
Protruding-mouth liver development having decreased processual parts cell population proliferation, abnormal
wnt2bbs403/s403; s854Tg control Long-pec endocrine pancreas size, normal
Long-pec liver decreased size, abnormal
Long-pec liver hypoplastic, abnormal
wnt2bbs403/s403; s854Tg + MO3-wnt2 standard conditions Long-pec endocrine pancreas size, normal
Long-pec extrahepatic duct decreased size, abnormal
Long-pec liver decreased size, abnormal
Long-pec liver development having decreased processual parts cell population proliferation, abnormal
Long-pec liver hypoplastic, abnormal
wnt2bbs404/s404 control Prim-5 liver decreased size, abnormal
Long-pec liver decreased size, abnormal
wnt2bbs404/s404 + MO3-wnt2 standard conditions Prim-5 liver absent, abnormal
Prim-5 liver decreased size, abnormal
Long-pec liver absent, abnormal
Long-pec liver decreased size, abnormal
wnt2bbs404/s404; gz2Tg; gz4Tg; s854Tg control Protruding-mouth extrahepatic duct decreased size, abnormal
Protruding-mouth gall bladder absent, abnormal
Protruding-mouth gall bladder decreased size, abnormal
Protruding-mouth liver decreased size, abnormal
Protruding-mouth liver development having decreased processual parts cell population proliferation, abnormal
Protruding-mouth liver hypoplastic, abnormal
wnt2bbs404/s404; gz2Tg; gz4Tg; s854Tg + MO3-wnt2 standard conditions Protruding-mouth extrahepatic duct absent, abnormal
Protruding-mouth extrahepatic duct decreased size, abnormal
Protruding-mouth gall bladder absent, abnormal
Protruding-mouth gall bladder decreased size, abnormal
Protruding-mouth liver absent, abnormal
Protruding-mouth liver development having decreased processual parts cell population proliferation, abnormal
wnt2bbs404/s404; s854Tg control Long-pec endocrine pancreas size, normal
Long-pec liver decreased size, abnormal
wnt2bbs404/s404; s854Tg + MO3-wnt2 standard conditions Long-pec endocrine pancreas size, normal
Long-pec extrahepatic duct decreased size, abnormal
Long-pec liver absent, abnormal
Long-pec liver decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development