ZFIN ID: ZDB-FIG-110909-14
Poulain et al., 2011 - Interplay between Wnt2 and Wnt2bb controls multiple steps of early foregut-derived organ development. Development (Cambridge, England)   138(16):3557-68 Full text @ Development
Anatomical Terms:
Stage Range: Prim-5 to Protruding-mouth

Fig. 3

Excess wnt2bb promotes liver formation at the expense of the ventral pancreas. (A-D2) Transient wnt2bb-overexpression in Tg(XlEef1a1:GFP)s854 (A-B2) and Tg(ptf1a:eGFP)jh1 (C-D2) embryos at 18 hpf alters foregut patterning resulting in an enlarged liver domain as visualised by Prox1 staining at 28 hpf (B, bracket: B2) and 48 hpf (D,D2). The pancreatic islet (asterisk) serves as landmark to visualize the posterior expansion of hepatoblast domain. (E-H) cp expression at 44 hpf (F) and Tg(fabp10:dsRed)gz4 expression at 74 hpf (H) indicate ectopic hepatic tissue differentiation in embryos with excess wnt2bb. Excess wnt2bb results in a severe decrease of pancreatic Tg(ptf1a:eGFP)jh1 (p: arrow, D) and Tg(ela3l:EGFP)gz2 expression (arrowhead, H). (A-D2,G,H) Ventral views of projections of confocal stacks; (E,F) dorsal views; all anterior towards the top.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cp WT control High-pec liver ISH
DsRed gz2Tg; gz4Tg control Protruding-mouth liver IFL
EGFP gz2Tg; gz4Tg control Protruding-mouth pancreas IFL
GFP jh1Tg control Long-pec pancreas IHC
s854Tg control Prim-5 digestive system endoderm IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab3-prox1 IHC jh1Tg control Long-pec liver
IHC s854Tg control Prim-5 liver primordium
Phenotype Details No data available
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development