ZFIN ID: ZDB-FIG-110909-14
Poulain et al., 2011 - Interplay between Wnt2 and Wnt2bb controls multiple steps of early foregut-derived organ development. Development (Cambridge, England)   138(16):3557-68 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Protruding-mouth

Fig. 3

Excess wnt2bb promotes liver formation at the expense of the ventral pancreas. (A-D2) Transient wnt2bb-overexpression in Tg(XlEef1a1:GFP)s854 (A-B2) and Tg(ptf1a:eGFP)jh1 (C-D2) embryos at 18 hpf alters foregut patterning resulting in an enlarged liver domain as visualised by Prox1 staining at 28 hpf (B, bracket: B2) and 48 hpf (D,D2). The pancreatic islet (asterisk) serves as landmark to visualize the posterior expansion of hepatoblast domain. (E-H) cp expression at 44 hpf (F) and Tg(fabp10:dsRed)gz4 expression at 74 hpf (H) indicate ectopic hepatic tissue differentiation in embryos with excess wnt2bb. Excess wnt2bb results in a severe decrease of pancreatic Tg(ptf1a:eGFP)jh1 (p: arrow, D) and Tg(ela3l:EGFP)gz2 expression (arrowhead, H). (A-D2,G,H) Ventral views of projections of confocal stacks; (E,F) dorsal views; all anterior towards the top.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cp WT control High-pec liver ISH
DsRed gz2Tg; gz4Tg control Protruding-mouth liver IFL
EGFP gz2Tg; gz4Tg control Protruding-mouth pancreas IFL
GFP jh1Tg control Long-pec pancreas IHC
s854Tg control Prim-5 digestive system endoderm IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab3-prox1 IHC jh1Tg control Long-pec liver
IHC s854Tg control Prim-5 liver primordium
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development