Fig. 3

Excess wnt2bb promotes liver formation at the expense of the ventral pancreas. (A-D2) Transient wnt2bb-overexpression in Tg(XlEef1a1:GFP)^s854 (A-B2) and Tg(ptf1a:eGFP)^jh1 (C-D2) embryos at 18 hpf alters foregut patterning resulting in an enlarged liver domain as visualised by Prox1 staining at 28 hpf (B, bracket: B2) and 48 hpf (D,D2). The pancreatic islet (asterisk) serves as landmark to visualize the posterior expansion of hepatoblast domain. (E-H) cp expression at 44 hpf (F) and Tg(fabp10:dsRed)^gz4 expression at 74 hpf (H) indicate ectopic hepatic tissue differentiation in embryos with excess wnt2bb. Excess wnt2bb results in a severe decrease of pancreatic Tg(ptf1a:eGFP)^jh1 (p: arrow, D) and Tg(ela3l:EGFP)^gz2 expression (arrowhead, H). (A-D2,G,H) Ventral views of projections of confocal stacks; (E,F) dorsal views; all anterior towards the top.