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ZFIN ID: ZDB-FIG-110621-5
Angotzi et al., 2011 - Involvement of Prop1 homeobox gene in the early development of fish pituitary gland. General and comparative endocrinology   171(3):332-340 Full text @ Gen. Comp. Endocrinol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 5

ISH on transverse sections of controls (Co; A–C) and prop1 MO (MO; D–F) injected embryos at 72 hpf using RNA probes for the pituitary lineage specific markers prl gh and tshβ. All three markers were strongly expressed in the pituitaries of both control and morphant embryos. Impaired cellular organization was observed for lactotrope (D vs. A) and somatotrope (E vs. B) lineages while thyrotropes were apparently unaffected by prop1 deficiency (F vs. C). Age in hpf and (treatments) are indicated in the upper-right hand corner; gene names in the lower-left corner. All panels are equally scaled (scale bar = 25 μm).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gh1 WT control Protruding-mouth somatotropin secreting cell ISH
WT + MO1-prop1 standard conditions Protruding-mouth somatotropin secreting cell ISH
prl WT control Protruding-mouth prolactin secreting cell ISH
WT + MO1-prop1 standard conditions Protruding-mouth prolactin secreting cell ISH
tshba WT control Protruding-mouth thyroid stimulating hormone secreting cell ISH
WT + MO1-prop1 standard conditions Protruding-mouth thyroid stimulating hormone secreting cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-prop1 standard conditions Protruding-mouth prolactin secreting cell spatial pattern, abnormal
WT + MO1-prop1 standard conditions Protruding-mouth somatotropin secreting cell spatial pattern, abnormal
WT + MO1-prop1 standard conditions Protruding-mouth thyroid stimulating hormone secreting cell spatial pattern, normal
Acknowledgments:
ZFIN wishes to thank the journal General and comparative endocrinology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Gen. Comp. Endocrinol.