ZFIN ID: ZDB-FIG-110121-29
Yu et al., 2011 - The Cell Adhesion-associated Protein Git2 Regulates Morphogenetic Movements during Zebrafish Embryonic Development. Developmental Biology   349(2):225-237 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage Range: 4-cell to Prim-5

Fig. 1 Identification and characterization of git2 genes in zebrafish. (A) Phylogenetic analysis of zebrafish git2 family genes. Dendogram of zebrafish git2a on chromosome 5 and git2b on chromosome 10 and related orthologs from other species. (B) In situ hybridization of git2a mRNA expression in the zebrafish embryo. git2a expression was ubiquitously detected at the 4-cell, epiboly, tailbud and 14-somite (14SS) and 24 hpf stages. (C) Western blotting of zebrafish Git2 protein at dome, 50%, 75%, 90% epiboly and 6-somite (6SS) stages, α-tubulin and paxillin were used as loading controls. (D) Immunohistochemistry of Git2 (red) at the 75% epiboly stage. Embryos were co-stained with phalloidin to detect F-actin (green). Images show surface EVL cells and deep cells (30 μm below the surface). Scale bar, 50 μm. Fluorescent intensity profiles show relative F-actin (green) and Git2 (red) levels in EVL cells at the blastoderm margin (1) and deep cells (2). (E) Immunohistochemistry of Git2 (red) at the 24 hpf. Embryos were co-stained with phalloidin to detect F-actin (green). Scale bar, 50 μm. Zebrafish embryo drawings are adapted from Kimmel et al., 1995.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
git2a AB standard conditions 4-cell whole organism ISH
75%-epiboly whole organism ISH
Bud whole organism ISH
14-19 somites whole organism ISH
Prim-5 whole organism ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-git IHC AB standard conditions 75%-epiboly DEL
IHC 75%-epiboly EVL cell-cell junction
IHC 75%-epiboly whole organism
IHC Prim-5 somite border
IHC Prim-5 whole organism
WB 50%-epiboly whole organism
WB Dome whole organism
WB 75%-epiboly whole organism
WB 90%-epiboly whole organism
WB 5-9 somites whole organism
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 349(2), Yu, J.A., Foley, F.C., Amack, J.D., and Turner, C.E., The Cell Adhesion-associated Protein Git2 Regulates Morphogenetic Movements during Zebrafish Embryonic Development, 225-237, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.