ZFIN ID: ZDB-FIG-110112-64
Bergeron et al., 2011 - Brother of cdo (umleitung) is cell-autonomously required for Hedgehog-mediated ventral CNS patterning in the zebrafish. Development (Cambridge, England)   138(1):75-85 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 4 boc morpholino (MO) injections cause uml-like defects. (A) A control MO-injected 48 hpf embryo. (B) uml mutants have curved body axes at this stage. (C) Injection of a translation blocking boc MO results in a curved body axis similar to that seen in uml mutants. (D) Normal nkx2.2a expression in the ventral forebrain and midbrain (arrowhead) of a control MO-injected embryo. (E) Regional loss of nkx2.2a expression seen in uml mutants (arrowheads). (F) Injecting boc MO results in the loss of nkx2.2a expression in the same regions as in uml mutants (arrowheads). (G) Zn-5 antibody labeling at 48 hpf showing RGC axons that have crossed the midline by 48 hpf to form the optic chiasm (arrowhead). (H) In uml mutants, RGC axons fail to cross the midline (arrowhead) and grow aberrantly in the ventral forebrain (arrows). (I) boc MO injections can result in specific retinal axon defects (arrowheads) that are remarkably similar to those seen in uml. A-C are lateral views of live zebrafish. D-F are lateral views of the head, eyes removed, anterior to the left. G-I are ventral views of the head, anterior uppermost.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
nkx2.2a WT control Prim-5 forebrain ventral region ISH
Prim-5 midbrain ventral region ISH
WT + MO1-boc standard conditions Prim-5 forebrain ventral region ISH
bocty54z/ty54z standard conditions Prim-5 forebrain ventral region ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zn-5 IHC WT control Long-pec cranial nerve II
IHC WT + MO1-boc standard conditions Long-pec cranial nerve II
IHC bocty54z/ty54z standard conditions Long-pec cranial nerve II
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-boc standard conditions Prim-5 midbrain ventral region physical object quality, abnormal
Long-pec axon extension involved in axon guidance process quality, abnormal
Long-pec cranial nerve II misrouted, abnormal
Long-pec post-vent region curved ventral, abnormal
Long-pec retinal ganglion cell axon guidance process quality, abnormal
Long-pec trunk curved ventral, abnormal
bocty54z/ty54z standard conditions Prim-5 midbrain ventral region physical object quality, abnormal
Long-pec axon extension involved in axon guidance process quality, abnormal
Long-pec cranial nerve II misrouted, abnormal
Long-pec post-vent region curved ventral, abnormal
Long-pec retinal ganglion cell axon guidance process quality, abnormal
Long-pec trunk curved ventral, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development