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ZFIN ID: ZDB-FIG-110112-63
Bergeron et al., 2011 - Brother of cdo (umleitung) is cell-autonomously required for Hedgehog-mediated ventral CNS patterning in the zebrafish. Development (Cambridge, England)   138(1):75-85 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-5

Fig. 2 Reduced Hh signaling in uml mutants. (A) The Hh target gene ptc1 (now known as ptch2) is expressed throughout the ventral CNS in wild-type embryos with a dorsal peak of expression in the zona limitans interthalamica (ZLI) (arrowhead). (B) In uml mutants, ptch2 expression is greatly reduced throughout the brain and spinal cord, and is nearly absent from the ZLI (arrowhead). (C) foxa2 is expressed in the ZLI (arrowhead) and ventral midbrain/hindbrain (bracket). (C2) In the spinal cord, foxa2 is expressed in the medial floor plate (FP, arrowhead) and lateral FP cells that correspond to the V3 region of the mammalian spinal cord (arrows). (D,D′) In uml mutants, foxa2 expression is reduced in the brain and is absent from the lateral floor plate (D′ arrows). (E,E′) nkx2.9 is expressed in the diencephalon (arrowhead in E), ventral midbrain/hindbrain (bracket) and in lateral FP cells (arrows in E′). (F,F′) In uml mutants, nkx2.9 expression is completely absent throughout the CNS. (G,G′) nkx2.2a is expressed in the diencephalon (arrowhead in G), ventral midbrain/hindbrain (bracket) and lateral floor plate of the spinal cord (arrows in G′). (H,H′) In uml mutants nkx2.2a expression is reduced in the diencephalon (arrowhead in H), ventral midbrain/hindbrain (bracket), and spinal cord (arrows in H′). (I,I′) olig2 is regionally expressed in the diencephalon (arrowheads) and in a band of cells in the spinal cord just dorsal to the floor plate (arrowhead in I′). (J,J′) In uml mutants, olig2 expression is regionally absent in the diencephalon (arrowheads in J) and expression is reduced and shifted ventrally in the spinal cord (arrowhead in J′). (K,K′) nkx6.1 is expressed in the ventral midbrain (arrowhead in K), hindbrain (bracket in K) and in the ventral half of the spinal cord (bracket with arrowhead in K′). (L,L2) In uml mutants, nkx6.1 expression is reduced in the brain (arrowhead and bracket in L) and shifted ventrally in the spinal cord (bracket with arrowhead in L′). (M,M′) pax7 is expressed in the dorsal midbrain (arrowheads in M), hindbrain (bracket in M), and spinal cord (bracket with arrowhead in M′). Inset shows pax3 expression. (N,N′) In uml mutants, pax7 (and pax3, inset) expression appears unchanged. A-N are lateral views of the heads of 24 hpf zebrafish, anterior to the left, eyes removed. Black dots mark the optic recess. C′-N′ are spinal cord cross-sections of embryos shown in C-N. In C′-N′, arrows mark the lateral floor plate and arrowheads mark the floor plate. In I′-N′, brackets with arrowheads mark the dorsoventral boundary of gene expression within the spinal cord. In C, D, M and N nkx2.2a labeling (red) was used to identify uml mutants. Upper right panel is a schematic representation of Hh target gene expression in the spinal cord of wild-type (left) and uml mutant (right) embryos. d, diencephalon; fb, forebrain; hb, hindbrain; mb, midbrain; sc, spinal cord; som, somites; t, telencephalon.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
foxa2 WT standard conditions Prim-5 hindbrain ventral region ISH
Prim-5 lateral floor plate ISH
Prim-5 medial floor plate ISH
Prim-5 midbrain ventral region ISH
Prim-5 zona limitans intrathalamica ISH
bocty54z/ty54z standard conditions Prim-5 hindbrain ventral region ISH
Prim-5 Not Detected lateral floor plate ISH
Prim-5 medial floor plate ISH
Prim-5 midbrain ventral region ISH
Prim-5 zona limitans intrathalamica ISH
nkx2.2a WT standard conditions Prim-5 diencephalon ISH
Prim-5 hindbrain ventral region ISH
Prim-5 lateral floor plate ISH
Prim-5 midbrain ventral region ISH
bocty54z/ty54z standard conditions Prim-5 diencephalon ISH
nkx2.9 WT standard conditions Prim-5 diencephalon ISH
Prim-5 hindbrain ventral region ISH
Prim-5 lateral floor plate ISH
Prim-5 midbrain ventral region ISH
bocty54z/ty54z standard conditions Prim-5 Not Detected central nervous system ISH
nkx6.1 WT standard conditions Prim-5 hindbrain ventral region ISH
Prim-5 midbrain ventral region ISH
Prim-5 spinal cord ventral region ISH
bocty54z/ty54z standard conditions Prim-5 hindbrain ventral region ISH
Prim-5 midbrain ventral region ISH
Prim-5 spinal cord ventral region ISH
olig2 WT standard conditions Prim-5 diencephalon ISH
Prim-5 spinal cord ISH
bocty54z/ty54z standard conditions Prim-5 diencephalon ISH
Prim-5 spinal cord ISH
pax3a WT standard conditions Prim-5 spinal cord dorsal region ISH
bocty54z/ty54z standard conditions Prim-5 spinal cord dorsal region ISH
pax7a WT standard conditions Prim-5 hindbrain dorsal region ISH
Prim-5 midbrain dorsal region ISH
Prim-5 spinal cord dorsal region ISH
bocty54z/ty54z standard conditions Prim-5 hindbrain dorsal region ISH
Prim-5 midbrain dorsal region ISH
Prim-5 spinal cord dorsal region ISH
ptch2 WT standard conditions Prim-5 forebrain ISH
Prim-5 hindbrain ISH
Prim-5 midbrain ventral region ISH
Prim-5 spinal cord ISH
Prim-5 zona limitans intrathalamica ISH
bocty54z/ty54z standard conditions Prim-5 forebrain ISH
Prim-5 hindbrain ISH
Prim-5 midbrain ventral region ISH
Prim-5 spinal cord ISH
Prim-5 Not Detected zona limitans intrathalamica ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
bocty54z/ty54z standard conditions Prim-5 regulation of smoothened signaling pathway process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development