header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-101123-40
Kim et al., 2010 - PDGF signaling is required for epicardial function and blood vessel formation in regenerating zebrafish hearts. Proceedings of the National Academy of Sciences of the United States of America   107(40):17206-17210 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Term:
Stage: Adult
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Adult

Fig. 5

PDGF signaling is required for coronary blood vessel formation during zebrafish heart regeneration. (A) Treatment of wild-type fish with DMSO or PDGFR inhibitor (inh.) from 2–14 dpa. snail2 ISH was performed using 10- and 14-dpa hearts. (Scale bar = 100 μm.) (B) Treatment of wild-type fish with DMSO or PDGFR inhibitor from 2–14 dpa. acta2 ISH was performed using 10- and 14-dpa hearts. (Scale bar = 100 μm.) (C) Treatment of fli1a:EGFP fish with PDGFR inhibitor from 2–14 dpa. Hearts were collected and processed at 14 dpa (DAPI staining, blue; tropomyosin staining, red). An example of blood vessel formation (green) in the regenerating hearts is marked by the yellow arrow. The dashed lines mark the approximate position of the amputation plane in AC. The white dashed line indicates the outline of the fibrin clot in the regenerating hearts. (Scale bar = 100 μm.) (D) Quantification of blood vessel formation. The total fluorescent (F.) area was measured relative to total clot area (n = 8 hearts for DMSO and PDGFR inhibitor treatment). *P < 0.0001.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
acta2 WT physical alteration: anatomical structure, chemical treatment: dimethyl sulfoxide Adult cardiac ventricle regenerating tissue ISH
WT physical alteration: anatomical structure, chemical treatment: pharmaceutical Adult cardiac ventricle regenerating tissue ISH
EGFP y1Tg physical alteration: anatomical structure, chemical treatment: dimethyl sulfoxide Adult cardiac ventricle regenerating tissue IFL
y1Tg physical alteration: anatomical structure, chemical treatment: pharmaceutical Adult Not Detected cardiac ventricle regenerating tissue IFL
snai2 WT physical alteration: anatomical structure, chemical treatment: dimethyl sulfoxide Adult cardiac ventricle regenerating tissue ISH
WT physical alteration: anatomical structure, chemical treatment: pharmaceutical Adult cardiac ventricle regenerating tissue ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg physical alteration: anatomical structure, chemical treatment: pharmaceutical Adult regenerating tissue blood vessel aplastic, abnormal
Adult regenerating tissue endothelial cell absent, abnormal
Adult tissue regeneration disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA