ZFIN ID: ZDB-FIG-100616-94
Insinna et al., 2010 - Analysis of a zebrafish dync1h1 mutant reveals multiple functions for cytoplasmic dynein 1 during retinal photoreceptor development. Neural Development   5:12 Full text @ Neural Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage Range: Long-pec to Day 5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 3 Morpholino knock-down of dync1h1 phenocopies cnb mutants. (A, B) Dorsal views and retinal histology of control (cntl) morpholino (MO) injected (A) and dync1h1 ATG MO injected (B) embryos shown at 3.5 dpf. Note the smaller eyes, expanded melanophores, and photoreceptor dysmorphogenesis. (C) Western blot of wild-type (WT) versus cnb embryos at 3, 4, and 5 dpf (left blot) and wild-type and dync1h1 morphants at 2 dpf (right blot). Each blot was cut and probed with either antisera against human DYNC1H1 (amino-terminal 321 amino acids; top) or gammaTubulin (bottom). Scale bar: 40 μm for retinal histology.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dync1h1 Ab1-dync1h1 WT standard conditions Long-pec whole organism WB
Ab1-dync1h1 Protruding-mouth whole organism WB
Ab1-dync1h1 Day 4 whole organism WB
Ab1-dync1h1 Day 5 whole organism WB
Ab1-dync1h1 WT + MO1-dync1h1 standard conditions Long-pec whole organism WB
Ab1-dync1h1 dync1h1mw20/mw20 standard conditions Protruding-mouth whole organism WB
Ab1-dync1h1 Day 4 whole organism WB
Ab1-dync1h1 Day 5 whole organism WB
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-dync1h1 standard conditions Protruding-mouth embryonic retina morphogenesis in camera-type eye process quality, abnormal
Protruding-mouth eye decreased size, abnormal
Protruding-mouth melanocyte distended, abnormal
Protruding-mouth photoreceptor cell morphogenesis process quality, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Neural Dev.