ZFIN ID: ZDB-FIG-100616-92
Insinna et al., 2010 - Analysis of a zebrafish dync1h1 mutant reveals multiple functions for cytoplasmic dynein 1 during retinal photoreceptor development. Neural Development   5:12 Full text @ Neural Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Observed In:
Stage Range: Day 4 to Day 5

Fig. 1 The cnb mutation disrupts photoreceptor development. (A-D) Side (A, C) and dorsal (B, D) views of 5-dpf wild-type (A, B) and cnb mutant (C, D) embryos. (E-F) Retinal histology of wild-type (E) and cnb (F) retina at 4 dpf. (G, H) Zpr-1 immunoreactivity (brown) to label double cone photoreceptors (PhR) in 4-dpf wild-type (G) and cnb embryos (H). (I, J) ID1 immunoreactivity (brown) to label rod photoreceptors in 4-dpf wild-type (G) and cnb embryos (H). Nuclei in (G, H) are counterstained with methylene blue. (K-N) Confocal microscopy of wild-type (K, M) and cnb (L, N) whole retina (K, L) and individual rod photoreceptor cells (M, N) from Tg(Xlrho:EGFP)fl1 fish. Green, Xlrho:EGFP-positive cells; red, propidium iodide positive nuclei. Scale bars: 1 mm in (A, C); 40 μm in (E-L); 5 μm in (M, N). EGFP, enhanced green fluorescent protein.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP dync1h1mw20/mw20; fl1Tg standard conditions Day 4 retinal rod cell IFL
fl1Tg standard conditions Day 4 retinal rod cell IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-rho IHC WT standard conditions Day 4 retinal rod cell
IHC dync1h1mw20/mw20 standard conditions Day 4 retinal rod cell
zpr-1 IHC WT standard conditions Day 4 retinal cone cell
IHC dync1h1mw20/mw20 standard conditions Day 4 retinal cone cell
Phenotype Details
Fish Conditions Stage Phenotype
dync1h1mw20/mw20 standard conditions Day 4 embryonic retina morphogenesis in camera-type eye process quality, abnormal
Day 4 photoreceptor cell morphogenesis process quality, abnormal
Day 4 retinal cone cell malformed, abnormal
Day 4 retinal rod cell malformed, abnormal
Day 5 embryonic retina morphogenesis in camera-type eye process quality, abnormal
Day 5 retina malformed, abnormal
dync1h1mw20/mw20; fl1Tg standard conditions Day 4 embryonic retina morphogenesis in camera-type eye process quality, abnormal
Day 4 photoreceptor cell morphogenesis process quality, abnormal
Day 4 retinal cone cell malformed, abnormal
Day 4 retinal rod cell malformed, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Neural Dev.