ZFIN ID: ZDB-FIG-100616-104
Insinna et al., 2010 - Analysis of a zebrafish dync1h1 mutant reveals multiple functions for cytoplasmic dynein 1 during retinal photoreceptor development. Neural Development   5:12 Full text @ Neural Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 13 Efficient transport of Rhodopsin(CT44)-GFP to the outer segment is disrupted with loss of Dync1h1 or Dctn1. Cryosections of 3.5-dpf retina from transgenic Tg(1.3xops:xRhoCT44-GFP) embryos in which the carboxy-terminal 44 amino acids of Rhodopsin are fused with GFP. Localization of GFP was evaluated in photoreceptors from (A) control morphants, (B) cnb mutants, (C) dync1h1 morphants or (D) dctn1a/b morphants. Upper row shows propidium iodide staining to highlight nuclei. Middle row shows RhoCT44-GFP. Bottom row shows color combined images. Arrowheads indicate cells with mislocalized GFP to the cell body region.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP a125Tg control Protruding-mouth retinal rod cell photoreceptor outer segment IFL
a125Tg + MO1-dync1h1 standard conditions Protruding-mouth retinal rod cell IFL
a125Tg + MO2-dctn1a + MO2-dctn1b standard conditions Protruding-mouth retinal rod cell IFL
dync1h1mw20/mw20; a125Tg standard conditions Protruding-mouth retinal rod cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
a125Tg + MO1-dync1h1 standard conditions Protruding-mouth photoreceptor cell morphogenesis process quality, abnormal
Protruding-mouth retinal rod cell photoreceptor outer segment physical object quality, abnormal
a125Tg + MO2-dctn1a + MO2-dctn1b standard conditions Protruding-mouth photoreceptor cell morphogenesis process quality, abnormal
Protruding-mouth retinal rod cell photoreceptor outer segment physical object quality, abnormal
dync1h1mw20/mw20; a125Tg standard conditions Protruding-mouth photoreceptor cell morphogenesis process quality, abnormal
Protruding-mouth retinal rod cell photoreceptor outer segment physical object quality, abnormal
Acknowledgments:
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