| ZFIN ID: ZDB-FIG-100616-104 |
| Gene: | |
|---|---|
| Fish: | |
| Knockdown Reagents: | |
| Anatomical Terms: | |
| Stage: | Protruding-mouth |
| Fish: | |
|---|---|
| Knockdown Reagents: | |
| Observed In: | |
| Stage: | Protruding-mouth |
Fig. 13 Efficient transport of Rhodopsin(CT44)-GFP to the outer segment is disrupted with loss of Dync1h1 or Dctn1. Cryosections of 3.5-dpf retina from transgenic Tg(1.3xops:xRhoCT44-GFP) embryos in which the carboxy-terminal 44 amino acids of Rhodopsin are fused with GFP. Localization of GFP was evaluated in photoreceptors from (A) control morphants, (B) cnb mutants, (C) dync1h1 morphants or (D) dctn1a/b morphants. Upper row shows propidium iodide staining to highlight nuclei. Middle row shows RhoCT44-GFP. Bottom row shows color combined images. Arrowheads indicate cells with mislocalized GFP to the cell body region. |
| Gene | Antibody | Fish | Conditions | Stage | Anatomy | Assay |
|---|---|---|---|---|---|---|
| GFP | a125Tg | control | Protruding-mouth | retinal rod cell photoreceptor outer segment | IFL | |
| a125Tg + MO1-dync1h1 | standard conditions | Protruding-mouth | retinal rod cell | IFL | ||
| a125Tg + MO2-dctn1a + MO2-dctn1b | standard conditions | Protruding-mouth | retinal rod cell | IFL | ||
| dync1h1mw20/mw20; a125Tg | standard conditions | Protruding-mouth | retinal rod cell | IFL |
| Acknowledgments: | This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Neural Dev. |
|---|