ZFIN ID: ZDB-FIG-100416-4
Wang et al., 2009 - Zebrafish beta-adrenergic receptor mRNA expression and control of pigmentation. Gene   446(1):18-27 Full text @ Gene
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage Range: Prim-5 to Prim-25

Fig. 5 Effects of adrb2a knock-down on atp5a1, atp5b, and tyr mRNA expression. MOs were injected into 1–4-cell stage embryos. The mRNA expression in wild-type embryos and in embryos injected with adrb2a MOs was quantified by qPCR at 26 and 36 hpf as the hypopigmentation phenotype induced by adrb2a MOs became identifiable at 36 hpf. (A) atp5a1 mRNA expression. (B) atp5b mRNA expression. (C) tyr mRNA expression. Data are presented as the means ± SEM of 12 independent experiments, after normalization to the expression of β-actin. ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
atp5f1b WT standard conditions Prim-5 whole organism RTPCR
Prim-25 whole organism RTPCR
WT + MO1-adrb2a standard conditions Prim-5 whole organism RTPCR
Prim-25 whole organism RTPCR
tyr WT standard conditions Prim-5 whole organism RTPCR
Prim-25 whole organism RTPCR
WT + MO1-adrb2a standard conditions Prim-5 whole organism RTPCR
Prim-25 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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Reprinted from Gene, 446(1), Wang, Z., Nishimura, Y., Shimada, Y., Umemoto, N., Hirano, M., Zang, L., Oka, T., Sakamoto, C., Kuroyanagi, J., and Tanaka, T., Zebrafish beta-adrenergic receptor mRNA expression and control of pigmentation, 18-27, Copyright (2009) with permission from Elsevier. Full text @ Gene