ZFIN ID: ZDB-FIG-091016-5
Wang et al., 2009 - Zebrafish beta-adrenergic receptor mRNA expression and control of pigmentation. Gene   446(1):18-27 Full text @ Gene
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-15 to Day 5

Fig. S4 Zebrafish β-ARs loss of function experiments. 1–4-cell stage embryos were injected with 6.7 ng (for adrb1 and adrb3a) or 1.7 ng (for adrb2b and adrb3b) of MO. (A) Zebrafish treated with the MO against adrb1 showed curved bodies, larger yolks and early larval lethality. (B–C). Zebrafish adrb2b MO induced embryo malformation. (B) adrb2b MO-injected living embryos grown to 32 hpf are shown. Note the abnormal body shape and the small size of the head. (C) The same embryos in panel (B) developed severe edema at 120 hpf. (D) adrb3a MOs had no observable effect on developing zebrafish embryos. adrb3a MO-injected living embryos grown to 60 hpf are shown. (E–F) Zebrafish adrb3b MO induced embryo malformation and early larval lethality. (E) adrb3b MO-injected living embryos grown to 32 hpf are shown. Note the malformations of the cervical region and the head. (F) Five of 14 embryos shown in panel (E) survived to 56 hpf. Note the abnormal shape of body and head.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-adrb1 standard conditions Protruding-mouth trunk curved, abnormal
Protruding-mouth whole organism viability, abnormal
Protruding-mouth yolk increased size, abnormal
WT + MO1-adrb2b standard conditions Prim-15 head decreased size, abnormal
Prim-15 trunk shape, abnormal
Day 5 whole organism edematous, abnormal
Day 5 whole organism swollen, abnormal
WT + MO1-adrb3a standard conditions Pec-fin whole organism morphology, normal
WT + MO1-adrb3b standard conditions Prim-15 head malformed, abnormal
Prim-15 vertebra 1 malformed, abnormal
Prim-15 vertebra 2 malformed, abnormal
Long-pec head shape, abnormal
Long-pec trunk shape, abnormal
Long-pec whole organism viability, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Gene for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Gene, 446(1), Wang, Z., Nishimura, Y., Shimada, Y., Umemoto, N., Hirano, M., Zang, L., Oka, T., Sakamoto, C., Kuroyanagi, J., and Tanaka, T., Zebrafish beta-adrenergic receptor mRNA expression and control of pigmentation, 18-27, Copyright (2009) with permission from Elsevier. Full text @ Gene