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General Information
ZFIN ID: ZDB-FIG-100413-8
Kim et al., 2005 - Wnt5 signaling in vertebrate pancreas development. BMC Biology   3:23 Full text @ BMC Biol.
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 1 Time-lapse imaging of insulin:GFP transgenic embryos shows cell migration defects in Fz-2 morphants. (A, C-H) Uninjected insulin:GFP transgenic embryo, (B, I-N) fz-2 MO-injected insulin:GFP transgenic embryo. All panels are dorsal views and anterior is to the left. Scale bar represents 100 μm. (A) Uninjected transgenic embryo, 24 hpf. (B) Fz-2 MO-injected transgenic embryo, 24 hpf. (C) At the 14-somite stage, bilateral patches of GFP-positive cells are visible in uninjected embryo. (D) At the 15–16 somite stage, GFP-positive cells have started proliferating. (E-G) At the 17 somite to 24 hpf stages, GFP-positive cells are aligned in bilateral rows of cells and undergo a medial and posterior migration. (H) At 24 hpf, all GFP-positive cells have merged to form one islet. (I) At the 14-somite stage, bilateral patches of GFP expression are apparent in fz-2 MO-injected embryos similar to uninjected embryos. (J-M) GFP-positive cells migrate in random directions in fz-2 morphant embryos. (N) At 24 hpf, GFP-positive cells have still not merged. (O) Trajectory of GFP-positive cells in uninjected insulin:GFP embryo. Notice that cells are uniformly moving posteriorly. (P) Trajectory of GFP-positive cells in fz-2 MO-injected insulin:GFP embryo. Notice cells are moving in random directions. A: anterior, P: posterior, T: time, L: left, R: right, O: origin.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP zf5Tg standard conditions Prim-5 insulin secreting cell IFL
Prim-5 posterior pancreatic bud IFL
zf5Tg + MO2-fzd2 + MO3-fzd2 standard conditions Prim-5 insulin secreting cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
zf5Tg + MO2-fzd2 + MO3-fzd2 standard conditions Prim-5 cell migration disrupted, abnormal
Prim-5 endocrine pancreas development disrupted, abnormal
ZFIN wishes to thank the journal BMC Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Biol.