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ZIRC
ZFIN ID: ZDB-FIG-051207-4
Kim et al., 2005 - Wnt5 signaling in vertebrate pancreas development. BMC Biology   3:23 Full text @ BMC Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 5 Wnt-5 and Fz-2 morphant embryos exhibit similar pancreatic islet defects at 24 hpf. In all panels, anterior is to the left and 24 hpf. .A-I, dorsal view; J-L, lateral view. (A, D, G, J) Wild-type embryos. (B, E, H, K) Fz-2 morphants. (C, F, I, L) Wnt-5 morphants. In situ hybridization analysis of (A, B, C) somatostatin, (D, E, F) glucagon, notice a hollow spot in the middle of each patch, (G, H, I) islet-1, (J, K, L) fspondin-2b. Note scattered pancreatic cells in Fz-2 and Wnt-5 morphants.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gcga WT standard conditions Prim-5 posterior pancreatic bud ISH
WT + MO2-fzd2 + MO3-fzd2 standard conditions Prim-5 posterior pancreatic bud ISH
WT + MO2-wnt5b standard conditions Prim-5 posterior pancreatic bud ISH
isl1 WT standard conditions Prim-5 posterior pancreatic bud ISH
WT + MO2-fzd2 + MO3-fzd2 standard conditions Prim-5 posterior pancreatic bud ISH
WT + MO2-wnt5b standard conditions Prim-5 posterior pancreatic bud ISH
spon1b WT standard conditions Prim-5 posterior pancreatic bud ISH
WT + MO2-fzd2 + MO3-fzd2 standard conditions Prim-5 posterior pancreatic bud ISH
WT + MO2-wnt5b standard conditions Prim-5 posterior pancreatic bud ISH
sst2 WT standard conditions Prim-5 posterior pancreatic bud ISH
WT + MO2-fzd2 + MO3-fzd2 standard conditions Prim-5 posterior pancreatic bud ISH
WT + MO2-wnt5b standard conditions Prim-5 posterior pancreatic bud ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-fzd2 + MO3-fzd2 standard conditions Prim-5 cell migration disrupted, abnormal
Prim-5 endocrine pancreas development disrupted, abnormal
WT + MO2-wnt5b standard conditions Prim-5 cell migration disrupted, abnormal
Prim-5 endocrine pancreas development disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal BMC Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Biol.