header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-051207-5
Kim et al., 2005 - Wnt5 signaling in vertebrate pancreas development. BMC Biology   3:23 Full text @ BMC Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 6 Wnt-5 and Fz-2 morphant embryos have other similar defects. In all panels, view is dorsal, anterior is to the left. (A-I, M-O) 3dpf, (J-L) 24 hpf stage. (A, D, G, J, M) Wild-type embryos. (B, E, H, K, N) Fz-2 morphants. (C, F, I, L, O) Wnt-5 morphants. In situ hybridization analysis of (A-C) insulin, (D-F) carboxypeptidase A, notice the hollow spot indicating the position of the islet, (G-I) ceruloplasmin, (J-O) pdx-1, (M) arrow, pdx-1-staining in islet.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cp WT standard conditions Protruding-mouth liver ISH
WT + MO2-fzd2 + MO3-fzd2 standard conditions Protruding-mouth liver ISH
WT + MO2-wnt5b standard conditions Protruding-mouth liver ISH
cpa5 WT standard conditions Protruding-mouth exocrine pancreas ISH
WT + MO2-fzd2 + MO3-fzd2 standard conditions Protruding-mouth exocrine pancreas ISH
WT + MO2-wnt5b standard conditions Protruding-mouth exocrine pancreas ISH
ins WT standard conditions Protruding-mouth endocrine pancreas ISH
WT + MO2-fzd2 + MO3-fzd2 standard conditions Protruding-mouth endocrine pancreas ISH
WT + MO2-wnt5b standard conditions Protruding-mouth endocrine pancreas ISH
pdx1 WT standard conditions Prim-5 pancreas primordium ISH
Protruding-mouth pancreas ISH
WT + MO2-fzd2 + MO3-fzd2 standard conditions Protruding-mouth pancreas ISH
WT + MO2-wnt5b standard conditions Prim-5 pancreas primordium ISH
Protruding-mouth pancreas ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-fzd2 + MO3-fzd2 standard conditions Protruding-mouth endocrine pancreas development disrupted, abnormal
WT + MO2-fzd2 + MO3-fzd2 standard conditions Protruding-mouth pancreas development disrupted, abnormal
WT + MO2-wnt5b standard conditions Protruding-mouth endocrine pancreas development disrupted, abnormal
Protruding-mouth pancreas development disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal BMC Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Biol.