ZFIN ID: ZDB-FIG-091217-121
Lang et al., 2009 - Basonuclin-2 requirements for zebrafish adult pigment pattern development and female fertility. PLoS Genetics   5(11):e1000744 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Days 21-29
PHENOTYPE:
Fish:
Observed In:
Stage: Days 21-29

Fig. 7 Pigment cell specification and early differentiation in bonaparte (bnc2) mutants.

Shown are corresponding views from the flanks of mid-metamorphic 8–10 SSL larvae with wild-type above and bonaparte (bnc2) mutants below. (A,B) Staining with L-dopa revealed previously cryptic cells that are competent to synthesize melanin (arrows) in both wild-type and mutant. (A,A′) Prior to staining. (B,B′) After staining. (C,C′) kit+ melanoblasts were detectable in both wild-type and bonaparte mutants. (D,D′) csf1r+ xanthophore precursors were found in both backgrounds. (E,E′) ednrb1+ cells that may be iridophores as well as other chromatophore precursors are present though fewer in bonaparte. (F,F′) purine nucleoside phosphorylase 1 (pnp1) expressing iridophore precursors (DMP unpublished data) are present though dramatically reduced in number in bonaparte.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
csf1ra WT standard conditions Days 21-29 trunk xanthophore ISH
bnc2utr16e1/utr16e1 standard conditions Days 21-29 trunk xanthophore ISH
ednrba WT standard conditions Days 21-29 trunk pigment cell ISH
bnc2utr16e1/utr16e1 standard conditions Days 21-29 trunk pigment cell ISH
kita WT standard conditions Days 21-29 trunk melanoblast ISH
bnc2utr16e1/utr16e1 standard conditions Days 21-29 trunk melanoblast ISH
pnp4a WT standard conditions Days 21-29 trunk iridophore ISH
bnc2utr16e1/utr16e1 standard conditions Days 21-29 trunk iridophore ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
bnc2utr16e1/utr16e1 standard conditions Days 21-29 pigmentation disrupted, abnormal
Days 21-29 trunk iridophore decreased amount, abnormal
Days 21-29 trunk pigment cell (sensu Vertebrata) decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.