ZFIN ID: ZDB-FIG-091217-123
Lang et al., 2009 - Basonuclin-2 requirements for zebrafish adult pigment pattern development and female fertility. PLoS Genetics   5(11):e1000744 Full text @ PLoS Genet.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 9 bnc2 promotes both early and late metamorphic stripe melanophores, but is required by fin melanophores only in the absence of csf1r and kit.

Shown on the left are wild-type (A), the single mutant phenotypes of kit (B), ltk (C), csf1r (D) and the double mutant phenotype of csf1r; kit (E). Shown on the right is bonaparte (bnc2) (F) and the corresponding double and triple mutants with bonaparte (G–J). Whole fish images as well as details of the flank are shown. kit mutants lack EM melanophores but retain LM melanophores; kit mutants and all combinations with kit also lack scale melanophores [e.g., arrowhead in (B)]. ltk mutants lack both LM melanophores and body iridophores, but retain EM melanophores. csf1r mutants lack LM melanophores and all xanthophores, but retain EM melanophores in a dispersed pattern. csf1r; kit double mutants lack nearly all hypodermal body melanophores and lack all xanthophores, but retain melanophores in the fins (inset). All double and triple mutant combinations with bonaparte exhibited fewer hypodermal melanophores than the corresponding non-compound mutants. Remaining body melanophores are almost entirely found in association with scales (e.g., arrowheads in H′). bnc2; csf1r; kit mutants lacked virtually all body chromatophores and exhibited only a few residual melanophores at the tips of the fins (arrow), as well as a “medallion” of iridophores over the cardiac region. Scale in (A), 5 mm for (A–J); scale in (A′), 2 mm for (A′–J′).

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
bnc2utr16e1/utr16e1 standard conditions Adult hypodermis melanocyte decreased amount, abnormal
Adult trunk melanophore stripe disorganized, abnormal
bnc2utr16e1/utr16e1; csf1raj4blue/j4blue standard conditions Adult hypodermis melanocyte decreased amount, abnormal
Adult trunk melanophore stripe disorganized, abnormal
bnc2utr16e1/utr16e1; csf1raj4blue/j4blue; kitab5/b5 standard conditions Adult hypodermis melanocyte decreased amount, abnormal
Adult melanophore stripe melanocyte decreased amount, abnormal
Adult scale melanocyte decreased amount, abnormal
Adult trunk melanophore stripe disorganized, abnormal
bnc2utr16e1/utr16e1; kitab5/b5 standard conditions Adult hypodermis melanocyte decreased amount, abnormal
Adult melanophore stripe melanocyte decreased amount, abnormal
Adult scale melanocyte decreased amount, abnormal
Adult trunk melanophore stripe disorganized, abnormal
bnc2utr16e1/utr16e1; ltkj9s1/j9s1 standard conditions Adult hypodermis melanocyte decreased amount, abnormal
Adult trunk melanophore stripe disorganized, abnormal
csf1raj4blue/j4blue standard conditions Adult hypodermis melanocyte decreased amount, abnormal
Adult hypodermis xanthophore absent, abnormal
Adult trunk melanophore stripe disorganized, abnormal
csf1raj4blue/j4blue; kitab5/b5 standard conditions Adult caudal fin melanocyte normal amount, normal
Adult caudal fin melanophore stripe present, normal
Adult hypodermis xanthophore absent, abnormal
Adult melanophore stripe melanocyte decreased amount, abnormal
Adult scale melanocyte decreased amount, abnormal
Adult trunk melanophore stripe disorganized, abnormal
kitab5/b5 standard conditions Adult melanophore stripe melanocyte decreased amount, abnormal
Adult scale melanocyte decreased amount, abnormal
Adult trunk melanophore stripe disorganized, abnormal
ltkj9s1/j9s1 standard conditions Adult hypodermis iridophore decreased amount, abnormal
Adult scale melanocyte decreased amount, abnormal
Adult trunk iridophore decreased amount, abnormal
Adult trunk melanophore stripe disorganized, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.