ZFIN ID: ZDB-FIG-091014-3
Kinkel et al., 2009 - Cyp26 enzymes function in endoderm to regulate pancreatic field size. Proc. Natl. Acad. Sci. USA   106(19):7864-7869 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 3

Cyp26 enzymes set the anterior limit of the insulin domain. (A) Wild-type insulin domain. (B) insulin is expanded in Cyp26a1-deficient gir mutants. (C–E) Knockdown of Cyp26b1 and Cyp26c1 singly or in combination does not expand insulin. (F and G) Knockdown of Cyp26b1 or Cyp26c1 in gir mutants expands the pancreas as with gir alone. (H) Deficiency in all 3 Cyp26 enzymes expands the pancreas more than with Cyp26a1 deficiency alone. All panels show 24 hpf, dorsal view, anterior to the left, 200x magnification. (I) Size of the insulin domain in response to Cyp26 deficiency. Note that the Cyp26a1 morpholino does not expand the pancreas as dramatically as in gir mutants. Wild-type, n = 19; a1MO, n = 10; b1MO, n = 34; c1MO, n = 28; b1 + c1 MO, n = 20; R115866, n = 10; gir + b1MO, n = 17; gir + c1MO, n = 11; gir + b1 + c1MO, n = 11.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ins WT control Prim-5 posterior pancreatic bud ISH
WT + MO1-cyp26b1 standard conditions Prim-5 posterior pancreatic bud ISH
WT + MO1-cyp26b1 + MO1-cyp26c1 standard conditions Prim-5 posterior pancreatic bud ISH
WT + MO1-cyp26c1 standard conditions Prim-5 posterior pancreatic bud ISH
cyp26a1rw716/rw716 control Prim-5 posterior pancreatic bud ISH
cyp26a1rw716/rw716 + MO1-cyp26b1 standard conditions Prim-5 posterior pancreatic bud ISH
cyp26a1rw716/rw716 + MO1-cyp26b1 + MO1-cyp26c1 standard conditions Prim-5 posterior pancreatic bud ISH
cyp26a1rw716/rw716 + MO1-cyp26c1 standard conditions Prim-5 posterior pancreatic bud ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-cyp26b1 standard conditions Prim-5 pancreas length, normal
WT + MO1-cyp26b1 standard conditions Prim-5 pancreas position, normal
WT + MO1-cyp26b1 + MO1-cyp26c1 standard conditions Prim-5 pancreas length, normal
Prim-5 pancreas position, normal
WT + MO1-cyp26c1 standard conditions Prim-5 pancreas length, normal
Prim-5 pancreas position, normal
cyp26a1rw716/rw716 control Prim-5 pancreas increased length, abnormal
Prim-5 pancreas mislocalised anteriorly, abnormal
Prim-5 pancreas development process quality, abnormal
cyp26a1rw716/rw716 + MO1-cyp26b1 standard conditions Prim-5 pancreas increased length, abnormal
Prim-5 pancreas mislocalised anteriorly, abnormal
Prim-5 pancreas development process quality, abnormal
cyp26a1rw716/rw716 + MO1-cyp26b1 + MO1-cyp26c1 standard conditions Prim-5 pancreas increased length, abnormal
Prim-5 pancreas mislocalised anteriorly, abnormal
Prim-5 pancreas development process quality, abnormal
cyp26a1rw716/rw716 + MO1-cyp26c1 standard conditions Prim-5 pancreas increased length, abnormal
Prim-5 pancreas mislocalised anteriorly, abnormal
Prim-5 pancreas development process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proc. Natl. Acad. Sci. USA for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA