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ZFIN ID: ZDB-FIG-090622-19
Miller et al., 2003 - Two endothelin 1 effectors, hand2 and bapx1, pattern ventral pharyngeal cartilage and the jaw joint. Development (Cambridge, England)   130(7):1353-1365 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-15 to Prim-25
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-15 to Prim-25

Fig. 8 hand2 represses joint and dorsal pharyngeal fates. Lateral views of wholemount wild type (A,D), hand2 mutant (B,E), hand2 mutant injected with Edn1-MOs (C) and edn1 mutant (F). (A-C) bapx1 expression at 36 hpf is present at its normal location and expanded ventrally in hand2 mutants (arrow). This expansion of bapx1 in hand2 mutants is edn1-dependent, as hand2 mutants injected with Edn1-MOs lack all first arch mesenchymal expression of bapx1, whereas the first pharyngeal pouch expression domain is present (C). We found no evidence for the converse relationship, as hand2 expression appeared unaffected in bapx1-MO-injected animals (data not shown). (D-F) eng2 expression at 30 hpf, localized to constrictor dorsalis, the dorsal first arch mesodermal core (see Kimmel et al., 2001b) expands ventrally in both hand2 and edn1 mutants. e, eye; pe, pharyngeal endoderm; pp1, pharyngeal pouch 1. Scale bars: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
en2a Df(Chr01:hand2)s6/s6 standard conditions Prim-15 constrictor dorsalis ISH
Prim-15 pharyngeal arch 1 mesoderm ISH
WT standard conditions Prim-15 constrictor dorsalis ISH
Prim-15 pharyngeal arch 1 mesoderm ISH
edn1tf216b/tf216b standard conditions Prim-15 constrictor dorsalis ISH
Prim-15 pharyngeal arch 1 mesoderm ISH
nkx3-2 Df(Chr01:hand2)s6/s6 standard conditions Prim-25 pharyngeal arch 1 mesenchyme ISH
Prim-25 pharyngeal pouch ISH
Df(Chr01:hand2)s6/s6 + MO1-edn1 standard conditions Prim-25 Not Detected pharyngeal arch 1 mesenchyme ISH
Prim-25 pharyngeal pouch 1 ISH
WT standard conditions Prim-25 pharyngeal arch 1 joint ISH
Prim-25 pharyngeal arch 1 mesenchyme ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
Df(Chr01:hand2)s6/s6 standard conditions Prim-15 embryonic viscerocranium morphogenesis disrupted, abnormal
Prim-15 mandibular muscle wholly dorsalized, abnormal
Prim-25 embryonic viscerocranium morphogenesis disrupted, abnormal
Prim-25 pharyngeal arch 1 mesenchyme physical object quality, abnormal
Df(Chr01:hand2)s6/s6 + MO1-edn1 standard conditions Prim-25 embryonic viscerocranium morphogenesis disrupted, abnormal
Prim-25 pharyngeal arch 1 mesenchyme physical object quality, abnormal
edn1tf216b/tf216b standard conditions Prim-15 embryonic viscerocranium morphogenesis disrupted, abnormal
Prim-15 mandibular muscle wholly dorsalized, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development