ZFIN ID: ZDB-FIG-090304-62
Feijoo et al., 2009 - Formation of posterior cranial placode derivatives requires the Iroquois transcription factor irx4a. Molecular and cellular neurosciences   40(3):328-337 Full text @ Mol. Cell Neurosci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-15 to Protruding-mouth

Fig. 7 irx4a is required for the correct development of the posterior lateral line. GFP expression was observed in the SqET20 (A, B), SqET4 (C, D) and cldnB:GFP (E, F) transgenic lines in control and morphant larvae at the indicated times post-fertilization. In situ hybridization was carried out using the eya1 (G, H) and sox2 (I, J) riboprobes. (A, B) Expression of GFP in 72 hpf control SqET20 transgenic line labels mantle cells (arrows in A, inset) and interneuromastic cells (inc). Morphant embryos (B) lack neuromast mantle cells but interneuromastic cells are present indicating that primordium migration occurred. (C, D) At 72 hpf mechanosensory hair cells (arrows in C, inset) are labeled in the SqET4 transgenic line while morpholino injected siblings (D) show the absence of these cells. (E, F) The migrating primordium, the trail of interneuromastic cells and deposited neuromasts express GFP in cldnB:GFP transgenic uninjected embryos (E). In contrast, MO4as injected cldnB:GFP embryos (F) show a decrease in the size of the migrating primordium and the absence of deposited neuromasts. Note that we have compared GFP label in a 32 hpf morphant with a 35 hpf control embryo. The primordium size difference is evident despite the fact that the primordium in the control fish has already deposited two neuromasts. (G–J) Expression of the primordium markers eya1 (G, H, insets show magnified views) and sox2 (I, J) shows a similar effect, the label is diminished in morphant embryos. inc, interneuromastic cells hc, hair cells; n, neuromast; pr, primordium. The entire panel shows lateral views of the trunk and tail region; dorsal is towards top, anterior is towards left. Scale bar in F: 50 μm for A–F, scale bar in J: 30 μm for G–J.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP sqet20Et control Protruding-mouth interneuromast cell IFL
Protruding-mouth neuromast mantle cell IFL
sqet20Et + MO5-irx4a standard conditions Protruding-mouth interneuromast cell IFL
Protruding-mouth Not Detected neuromast mantle cell IFL
sqet4Et control Protruding-mouth neuromast hair cell IFL
sqet4Et + MO5-irx4a standard conditions Protruding-mouth Not Detected neuromast hair cell IFL
zf106Tg control Prim-15 interneuromast cell IFL
Prim-15 posterior lateral line primordium IFL
zf106Tg + MO5-irx4a standard conditions Prim-15 posterior lateral line primordium IFL
eya1 WT control Prim-15 posterior lateral line primordium ISH
WT + MO5-irx4a standard conditions Prim-15 posterior lateral line primordium ISH
sox2 WT control Prim-15 posterior lateral line primordium ISH
WT + MO5-irx4a standard conditions Prim-15 posterior lateral line primordium ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Molecular and cellular neurosciences for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Molecular and cellular neurosciences, 40(3), Feijoo, C.G., Saldias, M.P., De la Paz, J.F., Gómez-Skarmeta, J.L., and Allende, M.L., Formation of posterior cranial placode derivatives requires the Iroquois transcription factor irx4a, 328-337, Copyright (2009) with permission from Elsevier. Full text @ Mol. Cell Neurosci.