Morpholino

MO5-irx4a

ID

ZDB-MRPHLNO-100416-8

Name

MO5-irx4a

Previous Names

Target

Sequence

5' - CAATCTGAGCATCTTACCTGAGGTG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

Splice blocking MO designed to bind to the donor splice site between exon 2 and intron 2.

Genome Resources

None

Target Location

Genomic Features

No data available

Expression

Gene expression in Wild Types + MO5-irx4a

Expressed Gene	Anatomy	Figures
egr2b	(not) hindbrain neural plate rhombomere 3 rhombomere 5	Fig. 2 , Fig. 3 from Feijoo et al., 2009
emx3	forebrain	Fig. 3 from Feijoo et al., 2009
eya1	posterior lateral line primordium preplacodal ectoderm	Fig. 4 , Fig. 7 from Feijoo et al., 2009
irx4a	(not) whole organism	Fig. 1 from Feijoo et al., 2009
myh6	presumptive atrium heart tube	Fig. 8 from Feijoo et al., 2009
myh7	presumptive cardiac ventricle heart tube	Fig. 8 from Feijoo et al., 2009
neurod1	anterior lateral line ganglion (not) epibranchial ganglion (not) posterior lateral line ganglion statoacoustic (VIII) ganglion	Fig. 4 , Fig. 5 from Feijoo et al., 2009
neurog1	neurogenic placode (not) posterior lateral line placode (not) trigeminal placode	Fig. 4 from Feijoo et al., 2009
pax2a	midbrain hindbrain boundary midbrain hindbrain boundary neural plate	Fig. 2 , Fig. 3 from Feijoo et al., 2009
pax8	otic placode	Fig. 5 from Feijoo et al., 2009
rx3	forebrain neural plate	Fig. 2 from Feijoo et al., 2009
six4b	preplacodal ectoderm	Fig. 4 from Feijoo et al., 2009
sox2	anterior neural plate (not) hindbrain neural plate posterior lateral line primordium	Fig. 2 , Fig. 7 from Feijoo et al., 2009

Phenotype

Phenotype resulting from MO5-irx4a

Phenotype	Fish	Figures
brain decreased size, abnormal	WT + MO5-irx4a	Fig. 1 from Feijoo et al., 2009
eye decreased size, abnormal	WT + MO5-irx4a	Fig. 1 from Feijoo et al., 2009
pericardium edematous, abnormal	WT + MO5-irx4a	text only from Feijoo et al., 2009
presumptive atrium heart tube increased size, abnormal	WT + MO5-irx4a	Fig. 8 from Feijoo et al., 2009
presumptive cardiac ventricle heart tube decreased size, abnormal	WT + MO5-irx4a	Fig. 8 from Feijoo et al., 2009
trigeminal ganglion neuron decreased amount, abnormal	WT + MO5-irx4a	Fig. 6 from Feijoo et al., 2009
whole organism dead, abnormal	WT + MO5-irx4a	text only from Feijoo et al., 2009

Phenotype of all Fish created by or utilizing MO5-irx4a

Phenotype	Fish	Conditions	Figures
presumptive cardiac ventricle heart tube decreased size, abnormal	WT + MO5-irx4a	standard conditions	Fig. 8 from Feijoo et al., 2009
eye decreased size, abnormal	WT + MO5-irx4a	standard conditions	Fig. 1 from Feijoo et al., 2009
brain decreased size, abnormal	WT + MO5-irx4a	standard conditions	Fig. 1 from Feijoo et al., 2009
pericardium edematous, abnormal	WT + MO5-irx4a	standard conditions	text only from Feijoo et al., 2009
presumptive atrium heart tube increased size, abnormal	WT + MO5-irx4a	standard conditions	Fig. 8 from Feijoo et al., 2009
trigeminal ganglion neuron decreased amount, abnormal	WT + MO5-irx4a	standard conditions	Fig. 6 from Feijoo et al., 2009
whole organism dead, abnormal	WT + MO5-irx4a	standard conditions	text only from Feijoo et al., 2009

Citations