ZFIN ID: ZDB-FIG-090113-27
Matsuda et al., 2009 - Interaction with Notch determines endocytosis of specific Delta ligands in zebrafish neural tissue. Development (Cambridge, England)   136(2):197-206 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 5-9 somites

Fig. 7 Mib1, Notch1a and Notch3 are not essential for DeltaC endocytosis. (A-C′) Distribution of DeltaC in wild-type zebrafish embryos (A,A′) and in two mib alleles, mibm178 (B,B′) and mibta5ab (C,C′). (D,D′) Distribution of DeltaC in Notch MO embryos. Eight-somite stage embryos were double stained with zebrafish DeltaC antibody zdC1 (grey in A-D, green in A′-D′) and β-catenin (red in A′-D′). A dorsal view of a lateral neurogenic domain in the caudal neural plate is shown. In WT embryos, only subset of cells express DeltaC in cytoplasmic puncta (A,A′). A neurogenic phenotype allows more cells to express DeltaC in both mib mutants and Notch MO embryos as Notch signaling is reduced (B-D). However, DeltaC distribution remains cytoplasmic in mibm178 (B,B′) and Notch MO embryos (D,D′), and it only accumulates on the cell surface in mibta52b mutant embryos (C,C′).

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-dlc IHC WT standard conditions 5-9 somites hindbrain neural plate cytoplasm
IHC WT + MO1-notch1a + MO1-notch3 standard conditions 5-9 somites hindbrain neural plate cytoplasm
IHC mib1m178/m178 standard conditions 5-9 somites hindbrain neural plate cytoplasm
IHC mib1ta52b/ta52b standard conditions 5-9 somites hindbrain neural plate cell surface
Ab3-ctnnb IHC WT standard conditions 5-9 somites hindbrain neural plate adherens junction
IHC WT + MO1-notch1a + MO1-notch3 standard conditions 5-9 somites hindbrain neural plate adherens junction
IHC mib1m178/m178 standard conditions 5-9 somites hindbrain neural plate adherens junction
IHC mib1ta52b/ta52b standard conditions 5-9 somites hindbrain neural plate adherens junction
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development