ZFIN ID: ZDB-FIG-090113-24
Matsuda et al., 2009 - Interaction with Notch determines endocytosis of specific Delta ligands in zebrafish neural tissue. Development (Cambridge, England)   136(2):197-206 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Bud

Fig. 4 The effect of loss of Notch receptors on surface accumulation of DeltaD. (A-C′) A comparison of DeltaD distribution in wild type, notch1a and notch3 morphants, and mibm178 mutant zebrafish embryos at the tail bud stage. Areas shown here approximately correspond to rectangular areas in Fig. S1A-D (see supplementary material). Embryos are double-stained with zdD2 (grey in A-C, green in A′-C′) and β-catenin (red in A′-C′). DeltaD-expressing cells are increased and DeltaD is mainly localized at cell surface in both Notch morphants (B,B′) and mibm178 mutants (C,C′). (D-F′) DeltaD distribution in Notch morphants with (F,F′) or without (E,E′) co-injection of deltaA and deltaD (deltaA&D) MOs. Though co-injection of deltaA&D MO dramatically reduces zdD2 staining (compare E with F), DeltaD is still localized at the cell surface.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-dld IHC WT standard conditions Bud hindbrain neural plate cytoplasm
IHC WT + MO1-dla + MO1-notch1a + MO1-notch3 + MO2-dld standard conditions Bud hindbrain neural plate cell surface
IHC WT + MO1-notch1a + MO1-notch3 standard conditions Bud hindbrain neural plate cell surface
IHC mib1m178/m178 standard conditions Bud hindbrain neural plate cell surface
Ab3-ctnnb IHC WT standard conditions Bud hindbrain neural plate adherens junction
IHC WT + MO1-dla + MO1-notch1a + MO1-notch3 + MO2-dld standard conditions Bud hindbrain neural plate adherens junction
IHC WT + MO1-notch1a + MO1-notch3 standard conditions Bud hindbrain neural plate adherens junction
IHC mib1m178/m178 standard conditions Bud hindbrain neural plate adherens junction
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development