ZFIN ID: ZDB-FIG-090113-22
Matsuda et al., 2009 - Interaction with Notch determines endocytosis of specific Delta ligands in zebrafish neural tissue. Development (Cambridge, England)   136(2):197-206 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Bud to 10-13 somites

Fig. 2 Accumulation of DeltaD protein on the plasma membrane in the mib mutant. (A,B) Hindbrain expression of deltaD transcript in wild-type (WT) and mibta52b 10-somite stage (10 ss) zebrafish embryos. (C,D) DeltaD protein in areas corresponding to the rectangles in A and B. Embryos are stained with zdD2. The patches of DeltaD expression in D are in rhombomeres 3, 4 and 5. (E,F) deltaD transcript in wild type and mibta52b at the tail bud stage (tb) in hindbrain neurogenic domains. Area shown here corresponds approximately to rectangular areas in Fig. S1A,B (see supplementary material). mibta52b embryos do not have significantly exaggerated deltaD expression. (G,H) zdD2 staining in corresponding wild-type and mibta52b neurogenic domains. In mibta52b mutants, most DeltaD is at the cell surface, even when expression is not so exaggerated.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dld WT standard conditions Bud hindbrain neural plate ISH
10-13 somites hindbrain ISH
mib1ta52b/ta52b standard conditions Bud hindbrain neural plate ISH
10-13 somites hindbrain ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-dld IHC WT standard conditions Bud hindbrain neural plate cytoplasm
IHC 10-13 somites hindbrain cytoplasm
IHC 10-13 somites rhombomere 3
IHC 10-13 somites rhombomere 4
IHC 10-13 somites rhombomere 5
IHC mib1m178/m178 standard conditions 10-13 somites hindbrain cytoplasm
IHC 10-13 somites rhombomere 3
IHC 10-13 somites rhombomere 4
IHC 10-13 somites rhombomere 5
IHC mib1ta52b/ta52b standard conditions Bud hindbrain neural plate cell surface
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development