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ZFIN ID: ZDB-FIG-081105-18
Lewis et al., 1999 - Control of muscle cell-type specification in the zebrafish embryo by hedgehog signalling. Developmental Biology   216(2):469-480 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to 26+ somites

Fig. 4 Expression of ptc1 at late somitogenesis stages in wild-type and mutant embryos. Specimens are oriented dorsal side up, anterior to the left unless otherwise stated. (A–G and J) 18- to 20-somite stage embryos; (H, I, and K–P) embryos at 26–30 somites. Specimens in E–G were stained with mAb 4D9 to reveal the expression of Eng proteins: arrows indicate Eng expression in MPs; square brackets indicate Eng expression in the midbrain/hindbrain region. (A) Wild-type expression of ptc1; (B) syu and (D) yot homozygotes showing strong reduction in ptc1 expression. The specimen in (C) showing a slight reduction in ptc1 expression is presumed to be a yot heterozygote. (F) con homozygote, identified by the lack of somitic Eng expression (compare to the wild-type sibling shown in (E)), exhibiting a strong reduction in ptc1 expression. (G and J) Details of embryos shown in (E and F) illustrating the simultaneous loss of ptc1 expression and loss of muscle pioneers in con homozygotes. Lateral (H and K) and dorsal (I and L) views of wild-type and you homozygous embryos showing reduction in expression of ptc1 in somites and neural tube, also shown at lower magnification in (M). (N) ubo homozygote at same stage as (M) showing normal expression of ptc1. (O) Lateral view of trunk region of a 26- to 30-somite stage wild-type embryo stained with mAb 4D9 to reveal Eng expressing MPs. Each somite has a small number (1–5) of cells that express high levels of Eng proteins located in the middle of the somite adjacent to the notochord. (P) ubo homozygotes do not form MPs but have clusters of cells expressing lower levels of Eng similar to those that surround MPs in wild-type embryos: note that this embryo was stained longer than that shown in (O) to reveal the lower level Eng staining.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
ptch2 WT standard conditions 14-19 somites to 20-25 somites muscle pioneer ISH
14-19 somites to 20-25 somites neural tube ventral region ISH
14-19 somites to 20-25 somites somite ISH
26+ somites neural tube ventral region ISH
26+ somites somite ISH
disp1tm15a/tm15a standard conditions 14-19 somites to 20-25 somites brain ISH
14-19 somites to 20-25 somites neural tube ventral region ISH
gli2aty119/ty119 standard conditions 14-19 somites to 20-25 somites brain ISH
14-19 somites to 20-25 somites neural tube ventral region ISH
prdm1atp39/tp39 standard conditions 26+ somites brain ISH
26+ somites neural tube ventral region ISH
26+ somites somite ISH
scube2ty97/ty97 standard conditions 26+ somites brain ISH
26+ somites neural tube ventral region ISH
26+ somites somite ISH
shhaDf(Chr07)t4/t4 standard conditions 14-19 somites to 20-25 somites brain ISH
14-19 somites to 20-25 somites neural tube ventral region ISH
14-19 somites to 20-25 somites Not Detected somite ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-eng IHC WT standard conditions 14-19 somites - 20-25 somites brain
IHC 14-19 somites - 20-25 somites muscle pioneer
IHC 26+ somites muscle pioneer
IHC disp1tm15a/tm15a standard conditions 14-19 somites - 20-25 somites brain
IHC 14-19 somites - 20-25 somites Not Detected muscle pioneer
IHC prdm1atp39/tp39 standard conditions 26+ somites adaxial cell
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 216(2), Lewis, K.E., Currie, P.D., Roy, S., Schauerte, H., Haffter, P., and Ingham, P.W., Control of muscle cell-type specification in the zebrafish embryo by hedgehog signalling, 469-480, Copyright (1999) with permission from Elsevier. Full text @ Dev. Biol.