ZFIN ID: ZDB-FIG-081105-19
Lewis et al., 1999 - Control of muscle cell-type specification in the zebrafish embryo by hedgehog signalling. Developmental Biology   216(2):469-480 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage: 26+ somites

Fig. 5 Expression of slow and fast myosin in wild-type and mutant embryos. Separated and merged immunofluorescence images of transverse sections of 26- to 30-somite stage embryos stained for fast MyHC (red) and slow MyHC (green). (A, F, and K) Wild-type embryo. Most of the slow-myosin-expressing cells have by this stage migrated to the lateral edge of the somite with the exception of the MPs that remain close to the notochord at the midline of the somite. (B, G, and L) yot homozygote; note the complete absence of all slow myosin expression. (C, H, and M) syu homozygote showing reduction in the number of cells expressing slow myosin; note that these have migrated correctly. (D, I, and N) you homozygote showing reduction in slow-myosin-expressing cells similar to that seen in syu. Note that some cells fail to migrate. (E, J, and O) A con homozygote showing normal distribution of slow muscle cells except for the absence of MPs (compare F and J).

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab-EB165 IHC WT standard conditions 26+ somites fast muscle cell
IHC disp1tm15a/tm15a standard conditions 26+ somites fast muscle cell
IHC gli2aty119/ty119 standard conditions 26+ somites fast muscle cell
IHC scube2ty97/ty97 standard conditions 26+ somites fast muscle cell
IHC shhaDf(Chr07)t4/t4 standard conditions 26+ somites fast muscle cell
Ab1-smyhc IHC WT standard conditions 26+ somites muscle pioneer
IHC 26+ somites slow muscle cell
IHC disp1tm15a/tm15a standard conditions 26+ somites Not Detected muscle pioneer
IHC 26+ somites slow muscle cell
IHC gli2aty119/ty119 standard conditions 26+ somites Not Detected muscle pioneer
IHC 26+ somites Not Detected slow muscle cell
IHC scube2ty97/ty97 standard conditions 26+ somites slow muscle cell
IHC shhaDf(Chr07)t4/t4 standard conditions 26+ somites slow muscle cell
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 216(2), Lewis, K.E., Currie, P.D., Roy, S., Schauerte, H., Haffter, P., and Ingham, P.W., Control of muscle cell-type specification in the zebrafish embryo by hedgehog signalling, 469-480, Copyright (1999) with permission from Elsevier. Full text @ Dev. Biol.