header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-081003-20
Yoshida et al., 2008 - Zebrafish orthologue of mental retardation protein IL1RAPL1 regulates presynaptic differentiation. Molecular and cellular neurosciences   39(2):218-228 Full text @ Mol. Cell Neurosci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 1-cell to Days 7-13

Fig. 1 Expression of zebrafish il1rapl genes. (A) Expression of zebrafish il1rapl1a, il1rapl1b and il1rapl2 mRNA in the 0 hpf, 24 hpf, 48 hpf and 72 hpf zebrafish embryos and 168 hpf larva estimated by RT-PCR. The sizes of il1rapl1a, il1rapl1b and il1rapl2 fragments were 825 bp, 828 bp and 211 bp, respectively. β-actin fragment of 490 bp served as a positive control. (B, C) Sections of 60 hpf embryos stained by in situ hybidization with DIG-labeled antisense (B) and sense (C) probes of the zebrafish il1rapl1a mRNA. (D, E) Sections of 60 hpf embryos stained by in situ hybidization with DIG-labeled antisense (D) and sense (E) probes of the zebrafish il1rapl1b mRNA. The dashed region in (D) including the olfactory placode is magnified on the left. eye, eye; ob, olfactory bulb; op, olfactory placode; tec, tectum; tel, telencephalon. Arrowheads point olfactory placodes. Scale bar, 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
il1rapl1a AB standard conditions 1-cell whole organism RTPCR
Prim-5 whole organism RTPCR
Long-pec to Days 7-13 whole organism RTPCR
Pec-fin optic tectum ISH
Pec-fin telencephalon ISH
il1rapl1b AB standard conditions 1-cell Not Detected whole organism RTPCR
Prim-5 whole organism RTPCR
Long-pec to Days 7-13 whole organism RTPCR
Pec-fin olfactory bulb ISH
Pec-fin optic tectum ISH
Pec-fin peripheral olfactory organ ISH
Pec-fin telencephalon ISH
il1rapl2 AB standard conditions 1-cell Not Detected whole organism RTPCR
Prim-5 Not Detected whole organism RTPCR
Long-pec to Days 7-13 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Molecular and cellular neurosciences for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Molecular and cellular neurosciences, 39(2), Yoshida, T., and Mishina, M., Zebrafish orthologue of mental retardation protein IL1RAPL1 regulates presynaptic differentiation, 218-228, Copyright (2008) with permission from Elsevier. Full text @ Mol. Cell Neurosci.