|ZFIN ID: ZDB-PUB-080801-5|
Your Input Welcome
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if additional information is required.
Oops. Something went wrong. Please try again later.
Zebrafish orthologue of mental retardation protein IL1RAPL1 regulates presynaptic differentiation
Yoshida, T., and Mishina, M.
|Source:||Molecular and cellular neurosciences 39(2): 218-228 (Journal)|
|Registered Authors:||Mishina, Masayoshi, Yoshida, Tomoyuki|
|PubMed:||18657618 Full text @ Mol. Cell Neurosci.|
Yoshida, T., and Mishina, M. (2008) Zebrafish orthologue of mental retardation protein IL1RAPL1 regulates presynaptic differentiation. Molecular and cellular neurosciences. 39(2):218-228.
ABSTRACTIL1-receptor accessory protein-like 1 (IL1RAPL1), a member of interleukine-1/toll receptor (TIR) family, is responsible for a nonsyndromic form of mental retardation (MR). The zebrafish orthologue of mammalian IL1RAPL1, designated as IL1RAPL 1B, was expressed widely in the brain and in the olfactory placode. We employed an olfactory sensory neuron-specific gene manipulation system in combination with in vivo imaging of transparent zebrafish embryos to examine the functional role of IL1RAPL 1B in synaptic vesicle accumulation and subsequent morphological remodeling of axon terminals, the characteristic features of presynaptic differentiation of zebrafish olfactory sensory neurons during synapse formation. Antisense morpholino oligonucleotide against IL1RAPL 1B suppressed both the synaptic vesicle accumulation and axon terminal remodeling. Consistently, the overexpression of IL1RAPL 1B stimulated synaptic vesicle accumulation. Swapping the carboxyl-terminal domain of IL1RAPL 1B with that of mouse IL-1 receptor accessory protein abolished the stimulatory effect. On the other hand, a substitution mutation in the TIR domain suppressed the morphological remodeling of axon terminals. Thus, the regulation of synaptic vesicle accumulation and subsequent morphological remodeling by IL1RAPL 1B appeared to be mediated by distinct domains. These results suggest that IL1RAPL 1B plays an important role in presynaptic differentiation during synapse formation.