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ZFIN ID: ZDB-FIG-080512-6
Marlow et al., 2004 - No tail co-operates with non-canonical Wnt signaling to regulate posterior body morphogenesis in zebrafish. Development (Cambridge, England)   131(1):203-216 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 5-9 somites to 14-19 somites

Fig. 4 Posterior tissues are specified in ppt;ntl and kny;ntl mutants. At nine somites, myoD is expressed in the somites and adaxial cells, and gta2 is expressed in two bilateral stripes of prospective blood in wild-type (A) embryos. In ntl mutants (B), adaxial cell expression of myoD is lost and the stripes of gta2 are less separated posteriorly. In ppt mutants (C), adaxial cell expression of myoD is kinked and gta2 is similar to the wild type. In kny mutants (D), somites are mediolaterally broader and the AP lengths of adaxial cells and the gta2 domain are shorter. The ppt;ntl (E) and kny;ntl (F) mutants exhibit myoD expression patterns as expected for the combined individual mutants, and gta2 stripes are fused posteriorly. (G-L) Expression of spt in the paraxial mesoderm of wild-type (G), ntl (H), ppt (I), kny (J), ppt;ntl (K) and kny;ntl (L) embryos. (M-R) Expression of Caudal in the tailbud of wild-type (M), ntl (N), ppt (O), kny (P), ppt;ntl (Q) and kny;ntl (R) embryos. (S-X) Expression of isl1 in developing neurons at the 16 somite stage. Ectopic isl1 expression in observed in ntl (T), ppt;ntl (W) and kny;ntl (X) mutants. (A-L) Dorsal posterior flat mounts. (M-X) Lateral views. Scale bars=100 μm (A-F,M-X), 50 μm (G-L).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cdx4 WT standard conditions 14-19 somites neuroectoderm ISH
14-19 somites tail bud ISH
gpc4m119/m119 standard conditions 14-19 somites neuroectoderm ISH
14-19 somites tail bud ISH
gpc4m119/m119; tbxtab195/b195 standard conditions 14-19 somites neuroectoderm ISH
14-19 somites tail bud ISH
tbxtab195/b195 standard conditions 14-19 somites neuroectoderm ISH
14-19 somites tail bud ISH
wnt5bta98/ta98 standard conditions 14-19 somites neuroectoderm ISH
14-19 somites tail bud ISH
wnt5bta98/ta98; tbxtab195/b195 standard conditions 14-19 somites neuroectoderm ISH
14-19 somites tail bud ISH
gata2a gpc4m119/m119 standard conditions 5-9 somites presumptive blood ISH
gpc4m119/m119; tbxtab195/b195 standard conditions 5-9 somites presumptive blood ISH
tbxtab195/b195 standard conditions 5-9 somites presumptive blood ISH
wnt5bta98/ta98 standard conditions 5-9 somites presumptive blood ISH
wnt5bta98/ta98; tbxtab195/b195 standard conditions 5-9 somites presumptive blood ISH
isl1 WT standard conditions 14-19 somites primary neuron ISH
gpc4m119/m119 standard conditions 14-19 somites primary neuron ISH
gpc4m119/m119; tbxtab195/b195 standard conditions 14-19 somites primary neuron ISH
tbxtab195/b195 standard conditions 14-19 somites primary neuron ISH
wnt5bta98/ta98 standard conditions 14-19 somites primary neuron ISH
wnt5bta98/ta98; tbxtab195/b195 standard conditions 14-19 somites primary neuron ISH
myod1 gpc4m119/m119 standard conditions 5-9 somites adaxial cell ISH
5-9 somites somite ISH
gpc4m119/m119; tbxtab195/b195 standard conditions 5-9 somites Not Detected adaxial cell ISH
5-9 somites somite ISH
tbxtab195/b195 standard conditions 5-9 somites Not Detected adaxial cell ISH
5-9 somites somite ISH
wnt5bta98/ta98 standard conditions 5-9 somites adaxial cell ISH
5-9 somites somite ISH
wnt5bta98/ta98; tbxtab195/b195 standard conditions 5-9 somites Not Detected adaxial cell ISH
5-9 somites somite ISH
tbx16 WT standard conditions 5-9 somites paraxial mesoderm ISH
gpc4m119/m119 standard conditions 5-9 somites paraxial mesoderm ISH
gpc4m119/m119; tbxtab195/b195 standard conditions 5-9 somites paraxial mesoderm ISH
tbxtab195/b195 standard conditions 5-9 somites paraxial mesoderm ISH
wnt5bta98/ta98 standard conditions 5-9 somites paraxial mesoderm ISH
wnt5bta98/ta98; tbxtab195/b195 standard conditions 5-9 somites paraxial mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development