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ZIRC
ZFIN ID: ZDB-FIG-080416-14
Huang et al., 2008 - Histamine metabolism influences blood vessel branching in zebrafish reg6 mutants. BMC Developmental Biology   8:31 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Term:
Stage: Adult

Fig. 8 Association of egr-1 expression and the reg6 vessel branching defect. (A) Expression levels of the egr-1 gene in wild-type (wt; black bars) and reg6 (white bars) 3-dpa regenerates. (B, C) Whole-mount in situ hybridization for egr-1 on wild-type (B) and reg6 (C) 3-dpa regenerates showed decreased expression of egr-1 in reg6 fish. Arrows, egr-1 RNA signal. (D) The egr-1 expression in reg6 mutants was upregulated more than 2.5-fold by 1 mM histamine and 10 μM SKF91488 but was downregulated by 300 μM urocanic acid. The expression level was determined by QPCR and normalized by the expression level of the β-actin gene.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
egr1 reg6j21/j21; y1Tg physical alteration: anatomical structure Adult regenerating fin ISH
y1Tg physical alteration: anatomical structure Adult regenerating fin ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.