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ZFIN ID: ZDB-FIG-080416-14
Huang et al., 2008 - Histamine metabolism influences blood vessel branching in zebrafish reg6 mutants. BMC Developmental Biology   8:31 Full text @ BMC Dev. Biol.
Anatomical Term:
Stage: Adult

Fig. 8 Association of egr-1 expression and the reg6 vessel branching defect. (A) Expression levels of the egr-1 gene in wild-type (wt; black bars) and reg6 (white bars) 3-dpa regenerates. (B, C) Whole-mount in situ hybridization for egr-1 on wild-type (B) and reg6 (C) 3-dpa regenerates showed decreased expression of egr-1 in reg6 fish. Arrows, egr-1 RNA signal. (D) The egr-1 expression in reg6 mutants was upregulated more than 2.5-fold by 1 mM histamine and 10 μM SKF91488 but was downregulated by 300 μM urocanic acid. The expression level was determined by QPCR and normalized by the expression level of the β-actin gene.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
egr1 reg6j21/j21; y1Tg physical alteration: anatomical structure Adult regenerating fin ISH
y1Tg physical alteration: anatomical structure Adult regenerating fin ISH
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.