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ZIRC
ZFIN ID: ZDB-FIG-080416-13
Huang et al., 2008 - Histamine metabolism influences blood vessel branching in zebrafish reg6 mutants. BMC Developmental Biology   8:31 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Terms:
Stage Range: Prim-15 to Adult
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Prim-15

Fig. 7 Blocking of histamine synthesis causes blood vessel branching defects in wild-type zebrafish embryos. (A) In a 32 hpf wild-type embryo, the developing caudal vein formed a transient vascular plexus. (B) When treated with 900 μM of the histamine synthesis blocker, urocanic acid, the caudal vein of a wild-type sibling formed much fewer vascular branches and even developed a reg6-like swollen lumen. Red arrows, caudal veins. (C) Quantitative results of the suppression of urocanic acid on blood vessel branching in wild-type zebrafish embryos. Two clutches of wild-type embryos (blue and red bars) were treated with the vehicle (0.0015 hydrochloric acid), or 300 or 900 μM of urocanic acid in egg water, from 26–36 hpf at 28.5°C. The percentage of embryos with less branched caudal vein was scored at the end of treatment (Y-axis, n = 40 unless otherwise specified).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP y1Tg standard conditions Prim-15 caudal vein plexus IFL
y1Tg chemical treatment: pharmaceutical Prim-15 caudal vein plexus IFL
egr1 reg6j21/j21; y1Tg physical alteration: anatomical structure Adult unspecified ISH
y1Tg physical alteration: anatomical structure Adult unspecified ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg chemical treatment: pharmaceutical Prim-15 caudal vein plexus morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.